DHA可抑制黄曲霉素B1诱导的肝癌细胞迁移及侵袭  

Docosahexaenoic acid inhibits aflatoxin B1-induced migration and invasion in hepatocellular carcinoma cells in vitro

作　　者：陈可和[1] 高婷[1] 潘金飞[1] 韦海明[1] 贾春宏[2] 蓝娇[1] 陈振祥[1] 潘登[1] 白晓春[2] 

机构地区：[1]广西壮族自治区人民医院化疗二区,广西南宁530021 [2]南方医科大学基础医学院,广东广州510515

出　　处：《南方医科大学学报》2016年第7期952-956,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81360368)~~

摘　　要：目的分析二十二碳六烯酸(DHA)对黄曲霉素B1(AFB1)诱导的肝癌细胞侵袭能力影响。方法不同浓度的AFB1、DHA/AFB1分别干预肝癌Hep G2.2.15细胞,通过细胞划痕、迁移及侵袭实验检测细胞迁移及侵袭能力变化,流式细胞仪分析细胞周期比例,透射电镜观察细胞超微结构的改变。结果 2μmol/L AFB1与空白组相比,细胞划痕修复率增高,细胞穿膜细胞数目均增多,G0/G1期细胞比例明显下降,G2/M期细胞比例明显升高,S期细胞比例变化不明显;细胞多核仁,细胞内出现线粒体、高尔基体增多等。DHA/AFB1与单独AFB1处理组的相比,细胞划痕修复率降低;细胞穿膜细胞数目均减少,G0/G1期细胞比例明显升高,G2/M期细胞比例明显降低,S期细胞比例变化不明显;细胞单核仁,细胞内核质减少,细胞内线粒体减少,出现空泡化超微结构。结论 DHA可以明显抑制AFB1诱导增强的Hep G2.2.15细胞迁移及侵袭能力。Objective To investigate the effect of docosahexaenoic acid（DHA） on invasiveness of aflatoxin B1（AFB1）-induced hepatocellular carcinoma cells in vitro.Methods Hep G2.2.15 cells were exposed to different concentrations of AFB1 and DHA plus AFB1.The cell migration and invasion were assessed using wound-healing and Transwell assay,and flow cytometry was used to analyze the cell cycle changes.The ultrastructural changes of the cells were observed by transmission electron microscopy.Results Compared with the control group,the cells exposed to2 μmol/L AFB1 showed obviously enhanced migration and invasion with decreased cell ratio in G0/G1 phase and increased cell ratio in G2/M phase but no changes in S phase cells; transmission electron microscopy revealed the presence of multiple nucleoli and significantly increased mitochondria and Golgi apparatus in the exposed cells.Compared with AFB1-exposed cells,the cells treated with DHA and AFB1 showed decreased migration and invasion abilities,and the G0/G1 phase cells increased and G2/M phase cells decreased significantly; ultrastructurally,the cells contained single nucleoli with decreased mitochondria and vacuolization occurred in the cytoplasm.Conclusion DHA can significantly inhibit AFB1-induced enhancement of cell migration and invasion in hepatocellular carcinoma cells in vitro.

关 键 词：肝癌细胞 黄曲霉素B1 DHA 

分 类 号：R735.7[医药卫生—肿瘤]