毛花甙丙抑制氧诱导乳鼠视网膜新生血管的实验研究  

Digilanid C inhibiting retinal neovascularization in oxygen induced retinopathy of mice

作　　者：张景尚[1] 安莹[1] 王进达[1] 李晓霞[1] 李婧[1] 熊瑛[1] 万修华[1] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科研究所北京市眼科学与视觉科学重点实验室,北京市100005

出　　处：《眼科新进展》2016年第7期612-614,共3页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30471861);国务院妇女儿童工作委员会课题(编号:2014108);北京市科技新星项目资助(编号:H020821380190;Z131102000413025)~~

摘　　要：目的探讨毛花甙丙对乳鼠氧诱导视网膜病变模型中视网膜新生血管的抑制作用。方法将20只生后第7天的C57乳鼠经体积分数75%±1%的氧诱导5 d后,分别给予左眼玻璃体内注射0.50μg(10眼)和0.10μg(10眼)毛花甙丙注射液,右眼注射相同剂量的磷酸盐缓冲液做对照,在生后第17天时进行视网膜铺片染色,计算视网膜新生血管面积和视网膜无灌注区面积,双眼间做配对样本t检验。结果给予0.05μg和0.10μg毛花甙丙玻璃体内注射后视网膜新生血管的面积分别为(1.09±0.32)mm^2和(0.92±0.40)mm^2,明显低于对照眼的(1.84±0.59)mm^2和(2.03±0.35)mm^2(均为P<0.05);0.05μg、0.10μg毛花甙丙注射后视网膜无灌注区面积分别为(1.40±0.44)mm^2和(1.07±0.40)mm^2,明显低于对照眼的(2.00±0.65)mm^2和(1.83±0.46)mm^2(均为P<0.05)。同时0.10μg毛花甙丙治疗效果优于0.05μg。结论毛花甙丙对氧诱导视网膜病变乳鼠模型中视网膜新生血管的生长具有抑制作用,同时还能促进视网膜无灌注区的修复。Objective To investigate the inhibitive effects of digilanid Con retinal neovascularization in the oxygen induced retinopathy of mice. Methods Seven-day-old C57 BL/6mice were exposed to 75% ± 1% oxygen for 5 days,and then returned to room air to induce retinal neovascularization. Twenty mice were divided into 2 groups equally. 0. 05μg and 0. 10 μg digilanid Cwere intravitreally injected into the left eye of each mouse in respective groups on postnatal day 12. Phosphate buffer saline was intravitreally injected into the right eye as control.Retinal neovascularization was evaluated by isolectin GS staining of retinal blood vessels on P17.T he area of neovascularization and obliteration were calculated,and the difference between digilanid Ceyes and the PBS eyes was analyzed by the paired t test. Results In both the 0. 05 μg group and 0. 10 μg group,the area of neovascularization in digilanid Ceyes were（ 1. 09 ± 0. 32）mm^2and（ 0. 92 ± 0. 40） mm^2,respectively,were significantly lower than that of the PBS eyes,which were（ 1. 84 ± 0. 59） mm^2and（ 2. 03 ± 0. 35） mm^2,respectively,there were statistical differences（ all P 〈 0. 05）. In both the 0. 05 μg group and 0. 10 μg group,the area of obliteration in digilanid Ceyes were（ 1. 40 ± 0. 44） mm^2and（ 1. 07 ± 0. 40） mm^2,respectively,were significantly low er than that of the PBS eyes,which were（ 2. 00 ± 0. 65） mm^2and（ 1. 83 ± 0.46） mm^2,respectively,there were statistical differences（ all P 〈 0. 05）. At the same time,the inhibitive effect of 0. 10 μg group was better than that of 0. 05 μg group. Conclusion Digilanid Cinhibits the retinal neovascularization in a mouse model of oxygen-induced retinopathy,and also can promote the repair of obliterative area.

关 键 词：毛花甙丙 氧诱导视网膜病变 视网膜病新生血管 鼠 

分 类 号：R774.1[医药卫生—眼科]