原发性肝癌患者外周血Vγ9Vδ2T细胞亚型及免疫功能分析  

Subtype analysis and feature detection of peripheral blood Vγ9Vδ2T cells in hepatocellular carcinoma patients

作　　者：马俊[1] 田伟[1] 贺杰峰[1] 任崇仁[1] 任晓静[1] 赵浩亮[1] 

机构地区：[1]山西医科大学附属山西大医院普外科,太原030032

出　　处：《中华普通外科杂志》2016年第7期549-552,共4页Chinese Journal of General Surgery

基　　金：山西省科技攻关基金资助项目（130313021-17）

摘　　要：目的检测肝癌（hepatocellularcarcinoma，HCC）患者与健康人外周血中Vy9V82T细胞比例，亚型以及免疫杀伤功能的变化。方法采集25例HCC患者和20例健康人的外周血应用流式细胞术检测82T细胞比例、分化成熟亚型、表达IFN-y和CDl07a比例；加入唑来膦酸和IL-2体外培养，12-14d后收集细胞再次检测上述指标。结果HCC患者外周血82T细胞占总T细胞比例培养前比健康人低（t=4．505，P〈0．001），经体外扩增后明显升高（t=8．782，P〈0．001），与健康组比较差异无统计学意义（t=1．644，P=0．109）。患者和健康人体内82T细胞分化亚型分布比较差异无统计学意义（均P〉0．05），而Tn、Tcm、Temra比例在培养后较培养前下降[t（Tn）=2．081，t（Tcm）=2．478，t（Temra）=2．953，均P〈0．05]，而Tem、Tem＋Temra较培养前明显升高[t（Tem）=12．6，t（Tern＋Temra）=9．843，均P〈0．001]。患者和健康人外周血中分泌IFN．1和CD107a的82T细胞比例在培养前后差异无统计学意义（P〉0．05）。结论肝癌患者外周血82T细胞比例低于健康人，成熟亚型、表达CD107a和IFN-y比例与健康人无差异，唑来膦酸加IL-2可以扩增肝癌患者与健康人82T细胞。Objective To investigate the percentage, mature classification and Immune killing function of Vγ9Vδ2Tcell in peripheral blood of HCC patients. Methods Peripheral blood mononuclear cells （PBMCs） were isolated from HCC patients （n = 25） and healthy donors （n = 20） by discontinuous density gradient centrifugation. Proportion, mature and differentiate subtypes and IFN-~/ and CD107a expressing of the delta 2 T cells were detect by using flow cytometry, ~2Teell were selectively cultured with zoledronate and human IL-2. After 12 - 14 days cells were collected and tested for the second time. Results While the percentage of Vγ9Vδ2TTcell of total T cell in peripheral blood of HCC patients is lower than healthy people before culture （ t = 4. 505, P 〈 0. 001 ） , after augmentation in vitro the proportion increased significantly （t =8. 782,P 〈0. 001 ） ,to a level similar to healthy group （ t = 1. 644,P =0. 109）. There was no statistically significant difference when differentiation subtypes of patient＇s Vγ9Vδ2T cell were compared with healthy group before culturing （ all P 〉 0. 05 ） , after culture the proportion of Tn, Tcm and Temra decreased [ t（Tn） = 2. 081 ,t（Tern） = 2. 478, t（Temra） = 2. 953, all P 〈 0. 05 ] , and the proportion of Tern, Tern ＋ Temra increased It（Tern） = 12. 6,t（Tem ＋ Temra） =9. 843,all P 〈0. 001 ]. Cell culture did not alter the proportion of IFN-Y and CD107a secreting Vγ9Vδ2T cells in the peripheral blood in both HCC patients and healthy people （ all P 〉 O. 05 ）. Conclusions While the percentage of Vγ9Vδ2Tcell of HCC patients in peripheral blood was lower than healthy people, its matured subtypes are similar to those of healthy people, and functions of expressing IFN-Y and CD107a are not different with healthy people. Applying ZOL ＋ IL-2 can amplify Vγ9Vδ2T cells of patients with HCC.

关 键 词：癌 肝细胞 T淋巴细胞 免疫疗法 

分 类 号：R735.7[医药卫生—肿瘤]