从空肠AQP3、GLUT1的表达变化探讨脾气虚、脾阳虚证的机理  

Mechanism of Expressions Changes of AQP3 and GLUT1 in Jejunum of Rats with Spleen-qi and Spleen-yang Deficiency Syndrome

作　　者：张林[1] 王艳杰[1] 王德山[1] 洪勇涛 杨晔[1] 冉博[1] 张文[1] 吴佳思 

机构地区：[1]辽宁中医药大学基础医学院,辽宁沈阳110847

出　　处：《辽宁中医杂志》2016年第7期1502-1504,I0002,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(2013CB531702);辽宁省教育厅高校科研基金(LJQ2014100)

摘　　要：目的:通过分析脾气虚、脾阳虚证模型大鼠空肠组织中水通道蛋白3、葡萄糖转运体1基因及蛋白的表达变化,从分子生物学角度,探讨空肠组织中AQP3及GLUT1与脾气虚、脾阳虚证之间的关系,为研究脾气虚、脾阳虚证分子机制提供新思路。方法:动物随机分为对照组、脾气虚组和脾阳虚组,复合因素造模;观察大鼠一般体征,运用Realtime PCR结合Western blot方法检测大鼠AQP3、GLUT1基因及蛋白表达变化。结果:脾气虚、脾阳虚模型大鼠下空肠组织中的AQP3、GLUT1基因及蛋白表达较对照组均有显著降低(P<0.01);阳虚组表达较气虚组也有显著降低(P<0.01)。结论:脾气虚、脾阳虚证状态下大鼠空肠AQP3、GLUT1表达下调,导致对水分和葡萄糖的吸收出现障碍,可能与脾气虚、脾阳虚证的发病机制有关。Objective： Through the analysis of gene and protein expressions of AQP3 and GLUT1 in jejunum of the rats with spleen- Qi deficiency syndrome and spleen- Yang deficiency syndrome based on the molecular biology to seek the relationship between these two syndromes and their differences and provide a new method about the molecular mechanism of spleen- Qi deficiency syndrome and spleen- Yang deficiency syndrome. Methods： Animals were randomly divided into three groups in terms of control group,spleen- Qi deficiency group and spleen- Yang deficiency group. Model rats were induced by combination of multi- methods and we observed physical signs,testified GLUT5 mRNA with Real- time PCR and protein with Western blot. Results：Compared with spleen- Qi deficiency and spleen- Yang deficiency,AQP3 and GLUT1 mRNA and protein were reduced obviously（ P〈0. 01）. Spleen- Yang deficiency syndrome＇s AQP3 and GLUT1 mRNA and protein reduced less than those of the spleen-qi deficiency syndrome（ P〈0. 01）. Conclusion： Down- regulated expressions of AQP3 and GLUT1 in jejunum of the rats may lead to malabsorption of water and glucose,which might be related with mechanism of spleen- Qi deficiency syndrome and spleen- Yang deficiency syndrome.

关 键 词：葡萄糖转运蛋白1 水通道蛋白3 脾气虚 脾阳虚 

分 类 号：R228[医药卫生—中医基础理论]