天麻川芎对自发高血压大鼠肾脏损伤及炎症因子的影响  

Effects of Tianma and Chuanxiong on Kidneys and Inflammation Factors in Spontaneous Hypertension Rats

作　　者：付彦君[1] 陶小军[2] 刘晴[3] 陈莹[3] 

机构地区：[1]辽宁中医药大学基础医学院基础药理教研室,辽宁沈阳110847 [2]辽宁中医药大学药学院药理教研室,辽宁大连116600 [3]辽宁中医药大学第一临床学院,辽宁沈阳110847

出　　处：《辽宁中医杂志》2016年第7期1528-1530,I0004,共4页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:研究天麻川芎(TC)对自发高血压大鼠肾脏损伤及炎症因子的影响。方法:自发高血压大鼠(SHR)分为SHR组、卡托普利组(90 mg·kg^(-1)·d^(-1))、TC大剂量组(18.4 g·kg^(-1)·d^(-1))和小剂量组(9.2 g·kg^(-1)·d^(-1)),WKY为正常对照。灌胃给药8周。给药结束后,化学比色法测24 h尿蛋白、免疫比浊法测尿微量白蛋白的含量;ELISA法测定血浆CRP水平;RT-PCR法测定肾脏TNF-α、IL-6、IL-1βmRNA表达水平;取肾脏做病理切片观察。结果:CT小、大剂量组均可显著降低SHR的24 h尿微量白蛋白水平,CT大剂量组可显著降低SHR的24 h尿蛋白水平;CT小、大剂量组均可显著降低SHR组的血浆CRP水平(P<0.05)及肾脏中的TNF-α、IL-6、IL-1βmRNA的表达水平,P值均小于0.05。肾脏病理切片显示CT使肾小球纤维化明显降低,炎细胞减少,肾小管纤维化减轻。结论:天麻川芎对自发高血压大鼠具有改善肾功能、减轻肾脏损伤的作用,其机制可能与降低CRP及炎症因子水平有关。Objective： To investigate the effects of Tianma and Chuanxiong on kidneys and inflammation factors in spontaneous hypertension rats（ SHR）. Methods： SHR rats were divided into 4 groups： SHR control,captopril（ 90 g·kg^-1·d^-1,large dose（ 18. 4 g·kg^-1·d^-1 and small dose（ 9. 2 g·kg^-1·d^-1. WKY rats were as normal control group. The medicines were given by i. g. for 8 weeks. The levels of microalbumin（ m- ALB） and total protein（ U- TP） in 24 h urine were detected by immunoturbidimetry and chemical colorimetry respectively. CRP in plasma was detected by ELISA. The expressions of mRNA of TNF- α,IL- 6 and IL- 1βmRNA in kidney were detected respectively by RT- PCR. Results： The levels of m- ALB and U- TP in 24 h urine were decreased markedly. The decline of the expressions of mRNA of TNF- α,IL- 6 and IL- 1βmRNA in kidneys of large and small CT group was significant and had histologic improvement compared with SHR group. Conclusion： Tianma and Chuanxiong can improve the kidney function,decrease the levels of CRP in serum and inflammatory factors mRNA in kidneys and reduce the degree of hypertensive renal damage.

关 键 词：天麻 川芎 自发高血压大鼠(SHR) 炎症因子 

分 类 号：R285.5[医药卫生—中药学]