Prdx1、Spy1、p27^(kip1)蛋白在肝细胞肝癌中的表达及其相关性  

The expressions and correlation between Prdx1 、Spy1 and p27^(kip1) proteins in hepatocellular carcinoma

作　　者：张婷婷[1] 倪润洲[1] 倪温慨[1] 刘肇修[1] 管程齐[1] 朱隽雅[1] 王靓[1] 王亚运[1] 仇惠元 

机构地区：[1]南通大学附属医院消化内科,江苏226001

出　　处：《交通医学》2016年第3期205-208,共4页Medical Journal of Communications

基　　金：国家自然科学基金面上项目(81472272);国家自然科学基金青年项目(81502070);南通大学研究生科技创新计划项目(YKS14013)

摘　　要：目的:探讨Prdx1、Spy1、p27^(kip1)蛋白在肝细胞肝癌中的表达及相关性。方法:应用Western blot及免疫组化检测肝癌及相应的癌旁组织中Prdx1、Spy1、p27^(kip1)的表达;免疫共沉淀分析Prdx1、Spy1的相互作用;Western blot分析Prdx1、Spy1相互结合对p27^(kip1)蛋白表达的影响。结果:Prdx1、Spy1在肝癌组织及肝癌细胞中高表达,与p27^(kip1)表达呈负相关;Prdx1、Spy1相互结合使p27^(kip1)的表达水平下调,此作用与Prdx1的抗氧化物活性无关。结论:Prdx1、Spy1蛋白的表达与肝细胞肝癌的发生发展有关,两者的表达可能在肿瘤的恶性进展中起协同作用,而与p27^(kip1)的表达呈拮抗作用。Objectiv e：To investigate the expressions and correlation between Prdx1, Spy1 and p27^kip1 proteins in hepatocellular carcinoma. Methods： Western blot and Immunohistochemical analysis were used to examine the expression of Prdx1, Spy1 and p27^kip1; immunoprecipitation assay was used to identify Prdx1 as a novel binding partner for Spy1. Results： Prdx1 and Spy1 expressions were observed in normal liver tissues distant from the tumors. It was also observed that partial co-localization of Prdx1 and Spy1 in HCC and Prdx1 interacted with Spy1 in liver tissue and HCC cells indicating that Prdx1 could potentially influence p27^kip1 function via Spy1. It was also showed that the antioxidant activity of Prdx1 was not essential for the formation of Prdx1/Spy1 complex. Conclusions： The abnormal expression of Prdx1 and Spy1 play possibly the promoting roles in HCC. The Prdx1 expression was significantly increased in HCC accompanying an increase in Spy1 levels and a decrease in p27^kip1 levels.

关 键 词：肝细胞肝癌 Prdx1蛋白 Spy1蛋白 P27KIP1蛋白 

分 类 号：R735.7[医药卫生—肿瘤]