采用高效液相色谱法检测余甘子中的没食子酸  

Determination of gallic acid in Phyllanthus emblica L. by high performance liquid chromatography

作　　者：郑伊琳 郑付春[2] 李春泉 李妙霞 陈潮升 叶丹彦[1] 陈沛洪 陈一村[1] 

机构地区：[1]汕头大学医学院药理教研室,广东汕头515041 [2]汕头大学医学院第一附属医院药剂科,广东汕头515041 [3]广东汇群中药饮片股份有限公司,广东汕头515041

出　　处：《癌变．畸变．突变》2016年第4期310-313,328,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：汕头市科技局科技计划项目[汕市财教(2014)242号];广东汇群特派员工作站建设(20150905)

摘　　要：目的：采用高效液相色谱法测定余甘子中没食子酸的含量,优化色谱条件,比较3个来源余甘子中没食子酸的含量差异。方法：分别对来自云南大理（2个批号）和福建漳州的余甘子中没食子酸进行含量测定,色谱条件为十八烷基硅烷键合硅胶色谱柱（粒径25μm,柱直径4.6 mm,柱长250 mm）;流动相乙腈-0.2%磷酸水溶液（5∶95）;等度洗脱;流速1.000 m L/min;检测波长2273nm;柱温30℃,标准曲线外标法。结果：没食子酸在13.010~130.104μg/m L范围内与峰面积有明显线性关系（R=0.999 4）;精密度良好[相对标准偏差（RSD）=0.08%];稳定性良好（RSD=0.29%）;重现性一般（RSD=4.23%）。对3批来自不同产地批号的余甘子药材进行了没食子酸含量评价,结果显示3个批次（福建20150304、云南20150205、云南20150304）的余甘子中没食子酸含量分别为2.60%、3.38%和2.82%,3个批次的余甘子中没食子酸含量均高于《中国药典》国家标准1.2%。结论：本方法测定余甘子中的没食子酸含量专属性强、精密度好、结果准确可靠。OBJECTIVE： To optimize chromatographic conditions for determination of gallic acid contents inPhyllanthus emblica L. from different regions. METHODS：Determination of gallic acid in Phyllanthus emblica L. indifferent regions was performed on an Eclipse plus C18 （25 μm,4.6 mm×250 mm）. The mobile phase was Acetonitrile-0.2% phosphoric acid solution （5∶ 95）. The elution was in same degree. The current speed was 1.000 mL/min. Thedetection wave length was 273 nm and the determination of linear regression equation used standard curve method. Thecolumn temperature was 30 degrees Celsius. RESULTS：Gallic acid had a good linear relationship in the range of2 13.010-130.104 g/mL （R =0.999 4）. It also had good precision （RSD=0.08%）,good stability （RSD=0.29%） and generalreproducibility （RSD=4.23%）. The content of gallic acid in the three batches of Phyllanthus emblica L. were higher thanChinese Pharmacopoeia national standard of 1.2%. CONCLUSION：The method for the determination of gallic acid inPhyllanthus emblica showed strong specificity and good precision. In addition,the results were accurate and reliable.

关 键 词：高效液相色谱法 余甘子 没食子酸 含量测定 

分 类 号：R917[医药卫生—药物分析学]