间歇性热应激预处理对小鼠行为学的影响  

Study on the effect of precondioning periodical heat stress on behavior of mice

作　　者：王衍[1] 陈玲[1] 金玉忠[1] 

机构地区：[1]沧州医学高等专科学校,河北沧州061000

出　　处：《临床和实验医学杂志》2016年第14期1349-1352,共4页Journal of Clinical and Experimental Medicine

基　　金：武警总部科研基金项目(编号:WK2003-12)

摘　　要：目的探讨间歇性热应激预处理对小鼠行为学的影响。方法复制小鼠间歇性热应激预处理模型,记录小鼠实验前后不同时间点的体重,用穿梭实验法,观察各组小鼠预处理后0 h、6 h、12 h、24 h、36 h不同时间点学习记忆功能。采用斜板实验法监测小鼠在间歇性热应激预处理后各时间点的脑体功能。结果 1与对照组相比,预处理20min、30 min组小鼠体重明显减轻(P<0.05)。与预处理20 min组相比,预处理30 min组体重明显减轻(P<0.05)。2穿梭实验结果:与对照组相比,预处理20 min组、30 min组在0 h,6 h点错误次数增加明显,潜伏期显著缩短(P<0.05)。间歇性热应激预处理20 min组小鼠在24 h点较对照组、30 min组,错误次数(M)明显减少,潜伏期(EL)明显延长(P<0.05)。3斜板实验结果表明:与对照组相比,在0 h、6 h时间点,预处理20 min组、30 min组斜板角度显著降低(P<0.05)。在24 h时间点,预处理20 min组斜板角度显著增高(P<0.05)。结论间歇性热应激预处理对小鼠的学习记忆以及脑体功能在一定时间内造成明显损伤,但是预处理20 min组小鼠24 h的学习记忆以及脑体功能出现明显的提高。Objective To explore the effect of preconditioning periodical heat stress on the behavior of mice. Methods Mice modelspreconditioning periodical heat stress had been duplicated. The weight of mice had been registered at different time points. Step - through test wasused to evaluate the ability of learning and memory at 0 h , 6 h , 12 h, 24 h and 36 h. The tiltboard test was applied to evaluate the physicalstrength of mice after preconditioning heat stress. Results ① In camparison with control group, the weight of mice at HP 20 min, 30 min wasmore lighter （ P 〈 0.05）. The weight of HP 30 min group is more lighter than that of HP 20 min group （ P 〈 0. 0 5 ） .② The step - through testindicated that HP 20 min and HP 30 min groups had more mistakes compared with those of control group while escape latency was shorter at 0 hand 6 h points （ P 〈 0. 05）. HP 20 min group has less mistakes and longer escape latency than those of control group and HP 30 min group at 24h point （ P 〈 0.05）. （3）The tiltboard test indicated that the degrees of decline in HP 20 min and 30 min groups at the time points Oh and 6h weresignificantly higher compared with those of control group （ P 〈0. 05）. While on the time of 24 h the degree of increase in HP 20 min group washigher （ P 〈 0.05）. Conclusion There is significant damnification on the behavior of mice after preconditioning heat stress. While it can be improvedin HP 20 min group at 24 h point.

关 键 词：小鼠 间歇性热应激 学习 记忆 

分 类 号：R-332[医药卫生] R33