SATB1在甲状腺癌启动PI3K/Akt信号通路中的作用研究  

The role of SATB1 in the initiation of PI3K/Akt signaling pathway in thyroid carcinoma

作　　者：龚龙[1] 许颖[2] 温飒飒 黄伟[1] 易春华[1] 童彦初[1] 陈文奎[1] 郑建伟[3] 

机构地区：[1]华中科技大学同济医学院附属荆州医院甲状腺血管外科,湖北荆州434020 [2]华中科技大学同济医学院附属荆州医院心内科,湖北荆州434020 [3]华中科技大学附属荆州医院附属同济医院胆胰外科,湖北武汉430030

出　　处：《临床和实验医学杂志》2016年第14期1352-1356,共5页Journal of Clinical and Experimental Medicine

基　　金：荆州市科技发展计划资助项目(2014031)

摘　　要：目的研究甲状腺癌细胞在发生、发展、侵袭及转移过程中富含AT序列的特异结合蛋白1(SATB1)所起的作用,探讨SATB1在启动PI3K/Akt信号通路中的作用。方法将甲状腺癌细胞随机分成4组:未处理组:除DMEM外不加任何处理;LY294002抑制组:DMEM培养集中加LY294002抑制剂10μmol/L;SATB1诱导剂组:DMEM培养集中加SATB1抗体200μg/L;TSATB1诱导抑制组:DMEM培养集中加SATB1L抗体200μg/L,加LY294002抑制剂10μmol/L。培养24 h后采集培养基中甲状腺癌细胞中血清采用酶联免疫(ELASE)法检测基质金属蛋白酶9(MMP9)、白细胞介素1(IL-1)和IL-6的表达情况,对甲状腺细胞裂解使用Western blotting方法测定PI3K、Akt、P-Akt及PIP3的表达情况,使用免疫组化方法检测甲状腺癌细胞中SATB1的表达。结果免疫组化方法可以看出:在甲状腺癌细胞胞核中SATB1表达为黄色,黄色越深表明表达越重,而在胞浆中无任何表达。不同实验组中SATB1的表达的深浅不一致且差异有统计学意义(P<0.05);其中SATB1诱导剂组蛋白SATB1的表达最高,表达最低的LY294002抑制组,且差异具有统计学意义(P<0.05)。Western blot研究证实,各组中PI3K、Akt、P-Akt及PIP3的表达不同且差异有统计学意义(P<0.05);其中SATB1诱导剂组PI3K、Akt、P-Akt及PIP3的表达最高,而LY294002抑制组最低(P<0.05)。ELASE法结果表明各组中MMP9、IL-1和IL-6的表达不同且差异有统计学意义(P<0.05);SATB1诱导剂组MMP9、IL-1和IL-6的表达最高,而LY294002抑制组最低(P<0.05)。结论 SATB1与甲状腺癌侵袭转移有关;SATB1在PI3 K/Akt信号通路具有启动的作用,同时SATB1可能参与肿瘤的微环境和炎性因子的表达。Objective To discuss role of the rich sequence specific binding protein 1 （ special at rich sequence binding protein 1,SATB1） in initiating PI3K/Akt signaling pathway on the occurrence of thyroid cancer cells as well as invasion and metastasis. Methods The culturedthyroid cancer cells were divided into 4 groups untreated group： in addition to DMEM without any treatment; LY294002 inhibited group：Cultured in DMEM with 10 [xmol/L inhibitor LY294002 ; SATB1 inducer group： DMEM medium plus SATB1 antibody 200 [xg/L; TSATB1 inducedinhibition of group ： DMEM medium plus SATB1L antibody 200 ｜xg/L and 10 [xmol/L inhibitor LY294002. After 24 hours of culture acquisitioncultivating base in thyroid carcinoma cells in serum by elase method to detect the expression of MMP9, IL - 1 and IL - 6 expression, on thecleavage of the thyroid cells using Western blotting were used to detect the expression of PDK, Akt, p - Akt and PIP3 , immunohistochemical detectioncell SATB1 expression. Results The expression of SATB1 in different experimental groups was detected by immunohistochemical method.The expression of SATB1 was mainly in the cytoplasm of yellow, which was not expressed in cytoplasm. SATB1 expression was different in differenttreatment groups and the difference was statistically significant （ P 〈0. 05）. The SATB1 induced agent histone SATB1 expression highest expressionof the lowest LY294002 inhibited group and the difference was statistically significant （ P 〈0. 05）. Western blot results showed that differentgroups of PI3K, Akt, p - Akt and PIP3 expression was varied and the difference has statistical significance（ P 〈 0.05）. The SATB1 induced agentgroup and the protein PDK, Akt, p - Akt and PIP3 expression was the highest, the lowest was LY294002 inhibited group and the differencewas statistically significant （ P 〈 0. 05）. Elase assays confirmed that different groups of MMP9 , IL - 1 and IL - 6 expression was varied and thedifference has statistical significance （ P 〈 0.05）. SATB1 in

关 键 词：甲状腺癌 SATB1 PI3K/AKT 

分 类 号：R736.1[医药卫生—肿瘤]