曲妥珠单抗联合紫杉醇诱导过表达HER-2的乳腺癌裸鼠皮下移植瘤乳腺癌细胞凋亡的作用机制和效果研究  

The discussion of the action mechanism and effect of trastuzumab combined with paclitaxel induced overexpression subcutaneous breast cancer xenografts in nude mice apoptosis in HER-2 breast cancer cell

作　　者：张丽华[1] 李静[1] 

机构地区：[1]山东省莱芜市人民医院肿瘤科,山东莱芜271199

出　　处：《临床和实验医学杂志》2016年第14期1373-1377,共5页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨曲妥珠单抗联合紫杉醇诱导过表达表皮生长因子受体-2(HER-2)的乳腺癌细胞凋亡的作用机制和治疗效果。方法通过建立HER2过表达乳腺癌裸鼠皮下移植瘤模型,并将其分为对照组、曲妥珠单抗组、紫杉醇组和曲妥珠单抗联合紫杉醇组,分别给予相对应的药物治疗21 d。统计分析各组裸鼠的肿瘤体积、瘤重和抑瘤率,并使用Annexin V/PI双染色法检测细胞凋亡率。使用免疫组化检测Ki-67、微血管密度(MVD)及血管内皮生长因子(VEGF)。结果曲妥珠单抗联合紫杉醇组的肿瘤体积增长最慢,在21 d时的体积远远小于其它各组(P<0.05);曲妥珠单抗联合紫杉醇组的瘤重最小,抑瘤率远远高于其它组(P<0.05);D组裸鼠的细胞凋亡率均高于A、B、C组,且差异有统计学意义(P<0.05);D组裸鼠的Ki-67、MVD密度及VEGF水平均显著低于其他各组(P<0.05)。结论曲妥珠单抗联合紫杉醇诱导过表达HER-2的乳腺癌细胞凋亡效果显著,可以有效抑制HER2过表达乳腺癌裸鼠皮下移植瘤的生长及血管生成,值得临床推广应用。Objective To discuss the action mechanism and effect of trastuzumab combined with paclitaxel induced overexpression breast cancer cell apoptosis in HER - 2 cells. Methods Establish HER2 over expression model of subcutaneous breast cancer xenografts in nude mice, which were divided into control group （ Group A） , trastuzmnab （ Group B） and paclitaxel （ Group C） and trastuzumab in combination with paclitaxel group （Group D） for the treatment 21 days. The tumor volume, tumor weight and the tumor inhibition rate were analyzed using Annexin V/PI double staining of apoptotie cell death rate. Ki - 67, MVD and VEGF were detected by immunohistoehemieal method. Results Trastuzumab in combination with paclitaxel group and the slowest growth in tumor volume, 21d volume was far smaller than the other groups （ P 〈 0.05 ） ; trastu- zumab in combination with paelitaxel group and the tumor weight of the smallest, the tumor inhibition rate was much higher than that in other roups （ P 〈0.05）. Group D cell apoptosis in nude mice P 〈 0.05 ）. Ki -67, MVD density and VEGF levels in sion tively was higher than those in A, B, C group, and the differenee was statistically significant Group D were significantly lower than those in the other groups （ P 〈 0.05 ）. Conclu- Trastuzumab in combination with paclitaxel induced apoptosis inhibit the growth of HER2 over expression and angiogenesis in in breast cancer cells overexpressing HEll- 2 effect significantly, can effec- subcutaneous breast cancer xenografts in nude mice, and it is worthy of clinieal application.

关 键 词：乳腺癌 裸鼠皮下移植瘤 过表达HER-2 曲妥珠单抗 紫杉醇 

分 类 号：R737.9[医药卫生—肿瘤]