沙塘鳢线粒体基因组全序列的测定和分析  

Sequencing and Analysis of the Complete Mitochondrial Genome of Odontobutis

作　　者：张燕萍[1] 郑红梅[1] 邵芳[2] 韩晓磊[1] 徐建荣[1] 顾志良[1] 

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500 [2]南京医科大学附属常州第二人民医院肿瘤研究所,江苏常州213003

出　　处：《常熟理工学院学报》2016年第4期97-104,共8页Journal of Changshu Institute of Technology

基　　金：教育部留学回国人员科研启动基金(第42批)"鳡鱼和赤眼鳟线粒体基因组及进化关系研究";苏州市科技基础设施建设项目"农业动物(畜禽;水产)种质研究重点实验室"(SZZD201001)

摘　　要：为揭示沙塘鳢(Odontobutis)线粒体基因组特征,采用PCR扩增和测序的方法获得沙塘鳢(Odontobutis)线粒体基因组全序列.通过生物信息学软件(t RNAscan-SE、RNAfold)定位沙塘鳢线粒体基因组基因结构及预测t RNA和r RNA的二级结构.沙塘鳢线粒体基因组全长16 930 bp,结构组成和其他硬骨鱼基本一致,包括13个蛋白质编码基因,22个t RNA,2个r RNA和1个D-loop区.13个蛋白质编码基因中,除了CO1是以GTG作为起始密码子,ND6以TTA作为起始密码子以外,其余都以ATG起始.沙塘鳢线粒体基因的终止密码子有3种类型,分别为完全密码子TAA、不完全密码子TA和T.除了t RNASer(AGN)和t RNACys外,其余20个t RNA基因的二级结构都是典型的三叶草结构.识别了沙塘鳢终止序列区、中央保守区D、E、F和保守序列区CSB-1、CSB-2和CSB-3.比较沙塘鳢cytb基因序列与其他物种同源性发现,沙塘鳢与平头沙塘鳢的同源性最高(87.03%),河川沙塘鳢次之(83.57%),与人的同源性最低(70.64%).该研究结果可为深入探讨沙塘鳢属及塘鳢科鱼类的系统分类提供依据,更真实地反映了沙塘鳢的分类地位和进化关系.The entire mitochondrial genome of Odontobutis was obtained by PCR based on conservative primers.It was showed that the mitochondrial genome sequence was 16, 930 bp long, including 13 protein genes, 22tRNA genes, 2 rRNA genes and a noncoding region. The base composition of the genome was A 30.0% , C28.9%, G 15.6% and T 25.5%. Protein-coding genes in Odontobutis mitochondrial genome used ATG as the initiation codons with the exception of CO1 which used GTG and ND6 used TAA. It was found that stop codons inOdontobutis included TAA, uncomplement codons TA and T. Most tRNA genes could form typical secondarystructures except tRNASer（AGN） and tRNACys. In addition, the D-loop region of Odontobutis mtDNA was 844 bp inlength. Compared with the mitochondrial DNA control region from other species, the extended terminal associated sequences, central conserved sequence blocks, conserved sequence blocks and putative promoters of the mitochondrial DNA control region in Odontobutis were observed. Sequence alignment of Cyt b genes of Odontobutisand other 10 species from GenBank showed that Odontobutis obscara and Odontobutis had the closest genetic relationship in the 11 species. A phylogenetic tree constructed using the Neighbor-Joining method indicated thatthe relationship among Sleeper Branch was close, the next were Sculpin Branch and Sciaenidae, and Cyprinidwas distantly related.

关 键 词：沙塘鳢 线粒体DNA序列 D-LOOP区 系统进化 

分 类 号：Q493[生物学—生理学] Q75