盐胁迫对贯叶连翘种子萌发及幼苗POD的影响  

The Effect of Salt Stress on Seed Germination and Seedling Growth of Hypericum perforatum L.

作　　者：胡真明[1,2] 张雅君[3] 沈天文 徐根娣[2] 江丽[2] 吕洪飞[2] 

机构地区：[1]浙江省永康市舟山镇林业站,浙江金华321308 [2]浙江师范大学化学与生命科学学院,浙江金华321004 [3]常熟理工学院生物与食品工程学院,江苏常熟215500

出　　处：《常熟理工学院学报》2016年第4期120-124,共5页Journal of Changshu Institute of Technology

基　　金：江苏省科技支撑计划项目"规模化生态鸡在循环经济体系中的应用及推广示范"(BE2012416)

摘　　要：为研究贯叶连翘的抗盐生理,本研究利用不同浓度的Na Cl溶液及相同浓度不同比例、不同盐分的混合溶液处理贯叶连翘种子进行种子萌发试验.结果表明:盐胁迫对贯叶连翘种子萌发具有抑制作用,其中Na_2CO_3的抑制作用最显著.采用垂直平板聚丙烯酰胺凝胶电泳,研究了贯叶连翘幼苗在不同浓度Na Cl溶液处理下不同时期过氧化物同工酶(POD)的变化情况.结果表明:不同时期的贯叶连翘在盐胁迫下,同工酶酶带有一定的差异.不同盐浓度下,同一时期贯叶连翘的同工酶酶带也有所差异.结论:盐胁迫对贯叶连翘种子萌发具有抑制作用,并对过氧化物同工酶(POD)有一定的影响.In order to understand the salt resistance of Hypericum perforatum L., and to prove whether it is suitable for saline alkali soil cultivation, the seed was treated by different concentration NaCl solution or mixed solution of same concentration with different salts. The result showed that salt stress inhibited H. perforatum seedgermination. The effect of Na2CO3 inhibition was the most significant in all salt treatments. The effect of saltstress on isoenzymes of POD in seeding growth of H. perforatum was analyzed through polyacrylamide gel electrophoresis. The results showed that there exist some differences in the isoenzymatic patterns of POD in differentstage. And some differences in the isoenzymatic patterns of POD also occurred in different salt concentrations onthe same stage. Therefore, a conclusion was reached that salt stress inhibited H. perforatum seed germinationand effect on Peroxidase isoenzyme （POD） activities of the seedling.

关 键 词：贯叶连翘 盐胁迫 种子萌发率 幼苗 过氧化物酶同工酶 

分 类 号：Q945.78[生物学—植物学]