海南砂仁对肝源性溃疡大鼠胃黏膜TFF1及TFF1 mRNA的影响  

Effect of amomun longiligulare on TFF1 and TFF1mRNA of gastric mucosa in rats with hepatogenic ulcer

作　　者：高湲[1] 孙涛[1] 谢毅强[1] 杨世忠[1] 

机构地区：[1]海南医学院

出　　处：《山西中医》2016年第7期52-54,共3页Shanxi Journal of Traditional Chinese Medicine

基　　金：海南省自然科学基金资助项目(编号:814302)

摘　　要：目的:观察海南砂仁对肝源性大鼠胃黏膜乳癌相关肽(TFF1)及TFF1mRNA蛋白表达的影响。方法:成年健康雄性SD大鼠50只,随机分为5组。除正常组外,其余四组在成功复制免疫损伤性肝纤维化大鼠模型的基础上,采用无水乙醇和阿司匹林灌胃法建立胃溃疡大鼠模型。1溃疡指数测定;2免疫组化和Real-time PCR检测实验大鼠胃黏膜TFF1和TFF1mRNA蛋白表达。结果:1除正常组外,其余各组大鼠溃疡面积及溃疡指数均较高于正常组,普萘洛尔组和海南砂仁低、高剂量组溃疡指数均低于模型组(P<0.05);2免疫组化和Real-time PCR:大鼠胃黏膜TFF1和TFF1mRNA蛋白表达模型组略高于正常组,普萘洛尔组、海南砂仁低、高剂量组均有明显提高,尤以海南砂仁高剂量组升高明显(P<0.05)。结论:海南砂仁对大鼠胃黏膜损伤具有保护作用,其机制可能与提高TFF1和TFF1mRNA蛋白表达有关。Objective： To observe on the effect of amomun longiligulare on expressions of relevant peptide（ TFF1） and TFF1 mRNA protein of gastric mucosa in rats with hepatogenic ulcer. Methods： 50 adult healthy male rats of SD were randomly divided into 5 groups. Except the normal group,the rest of 4 groups were made into models of rats with gastric ulcer by gavage of anhydrous alcohol and aspirin on the basis of successfully copied models of rats with hepatic fibrosis of immunologic injury. 1 The ulcer indexes were determined. 2 The expressions of TFF1 and TFF1 mRNA protein in gastric mucosa of rats were detected by immunohistochemistry and Real- time PCR. Result： 1In ulcer indexes： except the normal group,the ulcer area and ulcer indexes of rats in the rest of each group were higher than those in normal group; the ulcer indexes of Propranolol group and amomun longiligulare groups of low- and high- dose were lower than those of model group（ P 〈0. 05）. 2In the immunohistochemistry and Real- time PCR： the expressions of TFF1 and TFF1 mRNA protein of gastric mucosa of rats in model group were slightly higher than those in normal group; the expressions of Propranolol group and amomun longiligulare groups of low- and high- dose were significantly improved; especially,the expression of amomun longiligulare group of high- dose was more significantly enhanced（ P 〈0. 05）. Conclusion： Amomun longiligulare has a protective effect on injury of gastric mucosa in rats,and its mechanism may be related to the enhancement of expressions of TFF1 and TFF1 mRNA protein.

关 键 词：海南砂仁 肝源性溃疡 胃黏膜 TFF1 TFF1mRNA 实验研究 

分 类 号：R573[医药卫生—消化系统]