A Cyclometalated Iridium（Ⅲ） Complex Serves as a Phosphorescent Probe for Specific Mitochondrial Imaging in Living Cells  

作　　者：Jinquan Wang Xiaojuan Hou Chengzhi Jin Hui Chao 

机构地区：[1]Guangdong Provincial Key Laboratory of Biotechnology Candidate Drug Research, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China [2]Huaihua Medical College, Huaihua, Hunan 418000, China [3]MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, Guangdong 510275, China

出　　处：《Chinese Journal of Chemistry》2016年第6期583-588,共6页中国化学（英文版）

摘　　要：A new cyclometalated iridium（Ⅲ） complex [Ir（2-pq）2（HPIP）]C1 （lrl, 2-pq=3-phenylisoquinoline, HPIP= 2-（2-hydroxyphenyl）imidazo[4,5-f] 1,10-phenanthroline） was synthesized and applied to image mitochondria in living cells. Irl displayed excellent ability to selectively accumulate in mitochondria of live cells with no requirement of replacement of the culture medium. Due to Irl exhibiting better photostability than the commercially available mitochondrial trackers, this complex could be applicable for the imaging and tracking of the mitochondrial mor- phological changes over long periods of time. In addition, in vitro cytotoxicity investigation revealed that it! showed negligible cytotoxicity at the concentrations employed. Based on these, Irl is suitable for organelle-selective imaging in living cells.A new cyclometalated iridium（Ⅲ） complex [Ir（2-pq）2（HPIP）]C1 （lrl, 2-pq=3-phenylisoquinoline, HPIP= 2-（2-hydroxyphenyl）imidazo[4,5-f] 1,10-phenanthroline） was synthesized and applied to image mitochondria in living cells. Irl displayed excellent ability to selectively accumulate in mitochondria of live cells with no requirement of replacement of the culture medium. Due to Irl exhibiting better photostability than the commercially available mitochondrial trackers, this complex could be applicable for the imaging and tracking of the mitochondrial mor- phological changes over long periods of time. In addition, in vitro cytotoxicity investigation revealed that it! showed negligible cytotoxicity at the concentrations employed. Based on these, Irl is suitable for organelle-selective imaging in living cells.

关 键 词：iridium（Ⅲ） complex phosphorescent probe MITOCHONDRIA IMAGING 

分 类 号：Q244[生物学—细胞生物学] O614.825[理学—无机化学]