白花丹素对骨肉瘤MG-63细胞迁移的影响及其作用机制  

Effects of plumbagin on invasion in osteosarcoma cell line MG-63 and its possible mechanisms

作　　者：田林强[1] 刘晓潭[1] 郭志豪[1] 王宏伟[1] 张天吉[1] 

机构地区：[1]河南新乡医学院第三附属医院骨二科,新乡453003

出　　处：《中国癌症防治杂志》2016年第3期137-140,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：新乡医学院科学研究培育基金资助项目(2014QN104)

摘　　要：目的观察白花丹素对骨肉瘤细胞迁移的抑制作用并初步探讨其可能的机制。方法以2.5μmol/L、5μmol/L和10μmol/L浓度白花丹素分别作用于骨肉瘤MG-63细胞,以含0.1%DMSO完全培养基为对照组。作用24 h后,Transwell法观察骨肉瘤MG-63细胞迁移能力,Real-time PCR检测骨肉瘤MG-63细胞中Ezrin基因m RNA表达情况,Western blot检测Ezrin蛋白和p-Ezrin的表达情况。结果 Transwell结果显示,作用24 h后,各实验组下层小室细胞数明显减少,2.5μmol/L组、5μmol/L组和10μmol/L组的细胞迁移率分别为(69.9±4.5)%、(39.3±3.5)%和(26.2±4.1)%,呈浓度依赖性(P<0.05);Real-time PCR结果显示,骨肉瘤MG-63细胞中Ezrin m RNA表达水平明显下降,呈浓度依赖性(P<0.05)。Western blot结果显示,Ezrin蛋白和p-Ezrin的表达量明显降低,呈浓度依赖性(P<0.05)。结论白花丹素能够抑制骨肉瘤MG-63细胞迁移,其作用机制可能与抑制骨肉瘤MG-63细胞中Ezrin的表达及活化有关。Objective To assess the ability of plumbagin to inhibit invasion by osteosarcoma and explore possible mechanisms.Methods Osteosarcoma MG-63 cultures were treated for 24 h with 0.1% DMSO only（control） or with plumbagin at 2.5,5 and10 μmol/L. Then the invasive ability of the MG-63 cells was measured in a trans-well assay,changes in Ezrin m RNA levels were determined using real-time PCR,and changes in Ezrin and p-Ezrin protein levels were assessed using Western blot assay.Results Plumbagin treatment reduced the number of invading artificial basement cells in a dose-dependent manner,and rate of cell migration at the three plumbagin doses was respectively,69.9±4.5%,39.3±3.5% and 26.2±4.1% of control cells（P〈0. 05）.Real-time PCR showed that plumbagin significantly reduced level of m RNA in a dose-dependent manner（P〈0.05）. Similarly,Western blotting showed that plumbagin significantly reduced levels of Ezrin and p-Ezrin in a dose-dependent manner. Conclusion Plumbagin may inhibit tissue invasion by osteosarcoma MG-63 cells,and it may do so in part by suppressing Ezin and p-Ezrin expression.

关 键 词：骨肿瘤 骨肉瘤 白花丹素 EZRIN基因 MG-63细胞 迁移 

分 类 号：R738.1[医药卫生—肿瘤]