HPV感染宫颈组织中14-3-3σ表达及启动子甲基化水平检测  

Expression of 14-3-3σ and the detection of methylation of 14-3-3σ gene promoter in the cervix with HPV infection

作　　者：齐淑贞 王少明[2] 张瑞丽[3] 周斌兵[4] 尤志学[4] 苏晓红 王千秋 

机构地区：[1]中国医学科学院皮肤病医院,南京210042 [2]中国医学科学院肿瘤医院肿瘤研究所,北京100021 [3]南京医科大学附属无锡第二医院皮肤科,江苏无锡214002 [4]南京医科大学第一附属医院妇科,南京210029

出　　处：《中国麻风皮肤病杂志》2016年第7期405-409,共5页China Journal of Leprosy and Skin Diseases

基　　金：若干皮肤病性病省内诊疗体系建设与转化医学研究(编号:BL2012007)

摘　　要：目的:检测HPV感染宫颈组织中14-3-3σ启动子甲基化与14-3-3σmRNA的表达。方法:采用甲基化特异性PCR技术检测26例宫颈CIN3+宫颈原位鳞癌,39例宫颈尖锐湿疣及35例对照标本中14-3-3σ基因启动子区CpG岛甲基化状态,Q-PCR法检测14-3-3σmRNA表达,Western Blot检测14-3-3σ蛋白的表达。结果:宫颈CIN3+宫颈原位鳞癌组中14-3-3σ基因启动子区CpG岛甲基化率为73.08%,明显高于对照组的22.86%,差异有统计学意义(P<0.05);尖锐湿疣组为17.94%与对照组比较,差异无统计学意义(P>0.05)。宫颈CIN3+宫颈原位鳞癌组的14-3-3σmRNA及蛋白表达较对照组减少,宫颈尖锐湿疣组14-3-3σmRNA及蛋白表达较对照组增加。结论:HPV感染宫颈CIN3+宫颈原位鳞癌组织中14-3-3σ表达减少可能与14-3-3σ启动子高甲基化相关。Objective：To detect the level of methylation of 14-3-3σ gene promoter region, and the ex-pression of 14-3-3σ mRNA and protein in cervix with HPV infection. Methods：The levels of methylation of 14-3-3σ gene promoter CPG island,and the expression of 14-3-3σ mRNA and protein were detected by methylation-specific PCR, Q-PCR and western-blot respectively in 39 cases with cervical condyloma accumi-natum （ CA） , 35 controls and 26 cases with cervical intraepithelial neoplasia （ CIN3） complicated with cervi-cal squamous cell carcinoma （ SCC） . Results：The rate of the methylation of 14-3-3σgene promoter CPG is-land in the samples of SCC was 73.08%, which was higher than that in control group （22.86%）, with a sig-nificant difference （P〈0.05）. There was no significant difference in the rate between the samples of CA （17.94%） and of controls （P〉0.05）. The expression of 14-3-3σ mRNA and protein in the patients with CIN3 complicated with SCC was lower than that in controls （P〈0.05）. The expression of 14-3-3σ mRNA and protein in the patients with CA was higher than those in controls （P〈0.05）. Conclusion： The reduced expression of 14-3-3σin the patients with CIN3 complicated with SCC may be associated with the hypermeth-ylation of 14-3-3σ gene promoter region.

关 键 词：尖锐湿疣 人乳头瘤病毒 宫颈上皮内瘤变 宫颈原位鳞癌 甲基化 14-3-3σ 

分 类 号：R737.33[医药卫生—肿瘤]