Pt电极双酶生物传感器的研究和应用  

Pt electrode Pairs of Research and Application of Enzyme Biosensors

作　　者：蔡霞 

机构地区：[1]泰州市食品药品药检所,江苏泰州225300

出　　处：《广东化工》2016年第13期84-86,共3页Guangdong Chemical Industry

摘　　要：在双酶(乳酸脱氢酶、丙酮酸氧化酶)体系中加入乳酸时,在一定的范围内,随着乳酸浓度的增加,该体系发光强度不断增加。这是由于乳酸与乳酸脱氢酶在辅酶I的作用下,反应生成丙酮酸,丙酮酸同空气中的氧气在丙酮酸氧化酶的作用下反应产生的过氧化氢可以增敏鲁米诺的电化学发光。将双酶通过碳纳米管附载的方式修饰在电极表面上,得到的修饰电极对鲁米诺电化学发光产生的增敏作用是裸电极的2倍多,且修饰后的最低定量限为8.9×10-12 Mol/L。并将它应用于人体汗液中乳酸的测定及其运动员耐力训练水平的预测,得到的结果满意,重现性高,稳定性好,酶膜不容易脱落。Lactic acid（LA） could be oxidized under the catalysis of immobilized lactic dehydrogenase（LDH） and pyruvate oxidase（Py OD） to produce hydrogen peroxide with nicotinamide adenine dinucleotide（NAD） as coenzyme. The enzymatic generated hydrogen peroxide can enhance the ECL intensity of luminol. The ECL intensity responded upon the concentration of LA on modified electrode was 2 times than born electrode. The ECL-based biosensor showed excellent character for LA measurement. Its linear detection limit decrease to 8.9×10^-12 mol/L with relative standard deviation of 4.13 %（CLA 1.34×10^-10 mol/l, n=6） and average recovery of 101.3 %. This ECL-based LA biosensor has reached a lower detection limit. It can be applied to detect the concentration of LA in the sweat of athletes during the exercitation for evaluation of exercise degree, and get a good result.

关 键 词：乳酸 生物传感器 鲁米诺 

分 类 号：TQ[化学工程]