脐带间充质干细胞通过上调神经营养因子表达改善Aβ损伤大鼠的学习记忆能力  

Human umbilical cord mesenchymal stem cells protects against Aβ-induced impairment partly through up-regulation of expression of neurotrophins

作　　者：刘莎[1] 吴明[1] 施兵奇[1] 刘增娟[1] 侯艳宁[2] 

机构地区：[1]石家庄市第三医院药学部,河北石家庄050011 [2]中国人民解放军白求恩国际和平医院药学部,河北石家庄050082

出　　处：《中国药理学通报》2016年第7期980-985,共6页Chinese Pharmacological Bulletin

基　　金：河北省卫生厅资助项目(No 20150881)

摘　　要：目的观察人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,h UCMSCs)对β-样淀粉蛋白(amyloidβ,Aβ)损伤大鼠学习记忆能力的影响,并探讨神经营养因子(neurotrophin,NT)在h UCMSCs改善大鼠学习记忆能力中的作用。方法实验分5组,分别为:空白对照组(control,con)、Aβ溶剂对照组(v-con)、人脐带间充质干细胞对照组(h UCMSCs-con)、Aβ损伤组(injury),以及人脐带间充质干细胞治疗组(h UCMSCs)。双侧海马CA1区定位注射Aβ制备阿尔茨海默样学习记忆障碍大鼠模型;通过Morris水迷宫检测大鼠学习记忆能力;通过硫堇尼氏体染色观察海马CA1区细胞区形态;通过ELISA分析神经营养因子含量。结果侧脑室注射脐带间充质干细胞可改善Aβ损伤大鼠的空间学习记忆能力,对抗Aβ损伤大鼠海马CA1区锥体细胞损伤,增加Aβ损伤大鼠海马组织神经营养因子表达。结论脐带间充质干细胞可对抗Aβ的神经毒性作用,改善Aβ损伤大鼠的空间学习记忆能力,其神经保护作用与增加海马组织中神经营养因子表达有关。Aim To investigate the protective effect of human umbilical cord mesenchymal stem cells （ hUCM-SCs ） against Aβ-induced impairment in rats and the possible mechanism .Methods Male SD rats （ weight 210~230 g ） were divided randomly into five groups with ten in each：① control group （ con ）;② vehicle-control group （ sterile distilled water , v-con ）;③ hUC-MSCs-control group （ hUCMSCs-con ）;④ Aβinjury group （ injury ）;⑤ hUCMSCs treatment group （ hUCM-SCs） .Six-day Morris water maze behavioral task was employed to test the spatial learning and memory of the animals.Neuro-pathological evaluation under thionin stain was performed after behavioral task .The level of brain-derived neurotrophic factor （ BDNF ） and nerve growth factor （ NGF ） were tested through ELISA .Re-sults hUCMSCs enhanced the cognitive performance of Aβtreated rats, and reversed Aβ-induced cell loss in CA1 hippocampus.On the cellular level, hUCMSCs attenuated Aβinjection induced down-regulation of NGF and BDNF.Conclusion Administration of hUC-MSCs can reverse the behavioral and cellular impair-ment of Aβtreated rats, as well as the down-regulation of neurotrophins , thus exerting a neuronal protective effect, which provides a potential therapeutic strategy for disorders with learning and memory impairment , such as Alzheimer ’ s disease （ AD） .

关 键 词：人脐带间充质干细胞 阿尔茨海默病 β-样淀粉蛋白 神经营养因子 神经生长因子 脑源性神经营养因子 

分 类 号：R-332[医药卫生] R322.81