越鞠甘麦大枣汤对抑郁子代小鼠海马Akt及m-TOR分子表达的影响  

Influence of Yuejuganmaidazao Decoction on depression-like behavior and m-TOR and Akt expression in hippocampus of PPD offspring

作　　者：吴如燕 陆彩 陶伟伟[1] 夏宝妹[1] 薛文达[1] 任荔[1] 吴颢昕[1] 陈刚[1] 

机构地区：[1]南京中医药大学基础医学院中医脑病重点实验室,江苏南京210046

出　　处：《中国药理学通报》2016年第7期1022-1026,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81403041);江苏省高校优势学科支持项目(No PAPD);江苏省自然基金资助项目(No BK 20151568)

摘　　要：目的观察越鞠甘麦大枣汤对子代抑郁症的快速疗效,并分析其对Akt及m TOR分子的影响。方法成功建立产后抑郁子代模型后,随机分组如下:正常子代组(CTL-F1,8只),抑郁子代生理盐水组(Veh,8只)、越鞠甘麦大枣汤组(YG,8只)。Veh组给予生理盐水,YG组给予越鞠甘麦大枣汤(8.3 g·kg^(-1))。单次给药24 h后测量强迫游泳实验(FST)。取脑,Western blot法检测小鼠海马Akt及m-TOR的磷酸化及总体水平。结果 YG组的游泳不动时间比Veh组明显缩短(P<0.01),并且小鼠海马p-Akt和p-m TOR表达明显上调(P<0.05)。结论越鞠甘麦大枣汤可能通过上调Akt和m TOR表达,快速缓解子代抑郁样行为。Aim To observe the rapid antidepressant effect of Yuejuganmaidazao Decoction on postpatum de-pression offspring , and analyze its influence on the Akt and mTOR expression .Methods After postpartum de-pression model was established , the offsprings were randomly divided into the following groups： control group（CTL-F1,n =8）, vehicle group （Veh,n =8） and YG group （ YG, n =8 ） .Veh group was treated with vehicle , YG group was treated with Yueju gan-maidazao Decoction（8.3 g· kg -1 ）.Forced swimming test（FST） was measured 24 hours after single adminis-tration.The phosphorylation and total level of Akt and＆nbsp;m-TOR in the hippocampus was detected by Western blot.Results The immobility time in YG group was significantly shorter than that in Veh group （ P 〈0.01 ） , and the expression of p-Akt and p-mTOR in the hippocampus was significantly increased （ P 〈0.05 ） .Conclusion Yuejuganmaidazao Decoction may rapidly alleviate depression-like behaviors of PPD offsprings through upregulation of Akt and mTOR ex-pression .

关 键 词：产后抑郁子代 越鞠甘麦大枣汤 AKT mTOR 快速抗抑郁 氯胺酮 

分 类 号：R-332[医药卫生] R289.5