钙黏蛋白在地塞米松诱导的人牙髓细胞成牙本质向分化中的表达研究  

Expression of cadherins in dexamethasone- induced odontoblastic differentiation of human dental pulp cells

作　　者：邓子龙[1,2] 吴补领[1,2] 闫文娟[1,2] 屈茜[1,2] 陈明[1,2] 吕晓琳[1,2] 陈佳婧[1,2] 

机构地区：[1]南方医科大学南方医院,广东广州510515 [2]南方医科大学口腔医学院,广东广州510515

出　　处：《牙体牙髓牙周病学杂志》2016年第7期396-400,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金(81371137)

摘　　要：目的:探讨钙黏蛋白(cadherins)在地塞米松诱导的人牙髓细胞(HDPCs)成牙本质向分化过程中的作用。方法:酶消化法分离培养HDPCs,RT-PCR检测N-cadherin、OB-cadherin、R-cadherin在HDPCs中的表达;采用10-7mol/L地塞米松对第3代HDPCs进行处理,qRT-PCR检测成牙本质相关基因碱性磷酸酶(ALP)、牙本质基质蛋白-1(DMP-1)、牙本质涎磷蛋白(DSPP)和N-cadherin、OB-cadherin、R-cadherin表达水平的变化;茜素红染色观察矿化结节形成情况。结果:N-cadherin、OB-cadherin、R-cadherin均表达于HDPCs。地塞米松显著上调ALP、DMP-1、DSPP、R-cadherin的表达水平,同时显著下调N-cadherin、OB-cadherin,矿化结节形成情况则无明显差异。结论:地塞米松可诱导HDPCs向成牙本质细胞分化,同时调节钙黏蛋白的表达,提示钙黏蛋白可能参与调控地塞米松对HDPCs成牙本质向分化的诱导过程。AIM ：To investigate the effects of cadherins on dexamethasone -induced odontoblastic differ-entiation of human dental pulp cells （HDPCs）.METHODS：HDPCs were enzymatically separated from pulp tissues and cultured.The mRNA expression of N-cadherin,OB-cadherin and R -cadherin in HDPCs was determined by RT-PCR.The third passage of HDPCs were cultured in medium containing 10 -7 mol/L dexamethasone for 7 and 14 days respectively,and then qRT-PCR was performed to detect the expression changes of odontogenic -related genes,inclu-ding alkaline phosphatase （ALP）,dentin matrix protein （DMP -1）,dentin sialophosphoprotein （DSPP）and cadher-ins （N-cadherin,OB -cadherin and R -cadherin）.The formation of mineralized nodules was assessed using alizarin red staining at day 14.RESULTS：The mRNA of N-cadherin,OB-cadherin and R -cadherin was expressed in HD-PCs cultured in vitro.HDPCs grown in dexamethasone -containing medium showed a significant upregulation of ALP, DMP -1,R -cadherin and DSPP mRNA both at day 7 and 14,while the formation of mineralized nodules showed no difference.Meanwhile,the expression levels of N -cadherin and OB-cadherin were decreased.CONCLUSION：Dexamethasone can induce odontoblastic differentiation of HDPCs and modulate the expression of cadherins during this procedure,indicating that cadherins may play an important role in dexamethasone -induced odontoblastic differentia-tion of HDPCs.

关 键 词：地塞米松 钙黏蛋白 人牙髓细胞(HDPCs) 成牙本质分化 

分 类 号：R780.2[医药卫生—口腔医学]