3D激光扫描显微镜下前磨牙牙冠硬组织的微结构  

Microstructure of crown dental hard tissue of premolars observed by 3D laser scanning microscope

作　　者：许立侠[1] 徐红梅[1] 郭红延[1] 郭红梅[1] 孙丽梅[1] 

机构地区：[1]武警总医院口腔科,北京100039

出　　处：《牙体牙髓牙周病学杂志》2016年第7期423-426,430,共5页Chinese Journal of Conservative Dentistry

基　　金：武警总医院院内资助项目二类(WZ201024)

摘　　要：目的:运用3D激光扫描显微镜(3DLSM)观察分析前磨牙牙冠部釉质、牙本质及釉牙本质界(DEJ)处结构与功能的关系。方法:收集10个因正畸拔出的新鲜前磨牙,随机分为横剖面组(A组)和纵剖面组(B组),分别在3DLSM下进行激光彩色及3D图像观察。结果:釉质的激光彩色图中,A组釉柱晶体排列紧密呈鱼鳞状,釉柱间隙清晰可见,釉柱鞘弧形边界连续。B组釉柱晶体排列紧密呈条索状,釉柱鞘连续清晰。牙本质的激光彩色图中,A组牙本质小管开口管腔明显,管壁边缘为管周牙本质,其余为管间牙本质。B组牙本质小管呈条索状贯穿牙本质全层,沿途可见许多分支。DEJ的激光彩色图中,A、B两组均呈贝壳状,但A组凹凸形态较明显。3D图像则更清晰显示了A、B两组的立体结构形貌。结论:3D激光扫描显微镜下观察到的牙冠硬组织结构与其功能密切相关。AIM：To investigate the microstructure of enamel,dentin and dentin -enamel junction （DEJ）, and to analyze the relationship between the microstructure and the function of premolars by 3D laser scanning micro-scope（3DLSM）.METHODS：10 freshly extracted human premolars were divided into transverse section group（group A,n =5）and longitudinal section group （group B,n =5）and were examined under 3D LSM.RESULTS：In group A enamel surface morphology showed that the crystals of enamel rods were closely aligned,enamel rod sheath gap was clearly visible and curved boundary was continuous.While in group B,the crystals of enamel rods were closely ar-ranged,enamel rod sheath was clear and continuous.For dentin surface morphology,many openings of dentin tubule could be found,lumen was clear and tube wall was surrounded with peritubular dentin and the other parts were surroun-ded with intertubular dentin in group A.Whereas in group B,dentin tubule was streakily arranged throughout the dentin layer,and was lined with many branches.As for surface morphology of DEJ,hydroxyapatite crystals formed a scalloped shape with its convexities directed toward the dentin and concavities directed toward the enamel in both groups,but the shape was more apparent in group A.CONCLUSION：The organizational structure of crown dental hard tissue is closely related to its function.

关 键 词：釉质 牙本质 釉牙本质界 3D激光扫描显微镜 

分 类 号：R780.41[医药卫生—口腔医学]