苯妥英钠对大鼠骨髓间充质干细胞向血管内皮细胞分化的影响  

The effects of phenytoin on the differentiation of rat bone marrow mesenchymal stem cells into vascular endothelial cells

作　　者：何珊[1] 王宝彦[2] 

机构地区：[1]西安交通大学医学院第二附属医院口腔科,710004 [2]西安交通大学口腔医院牙周粘膜科

出　　处：《实用口腔医学杂志》2016年第4期457-461,共5页Journal of Practical Stomatology

基　　金：陕西省科技厅社发攻关项目(编号:2012SF2-22)

摘　　要：目的:研究苯妥英钠(PHT)对大鼠骨髓间充质干细胞(rBMSCs)向血管内皮细胞(rVECs)分化的影响。方法:建立rBMSCs与rVECs共培养组及rBMSCs单独培养组,各组分别添加不同浓度的PHT(0、20、40μg/ml),培养14 d后real-time PCR检测各组细胞中ICAM-1、VCAM-1、KDR和RUNX2基因的mRNA表达水平。结果:添加PHT后,各组细胞中ICAM-1、KDR和RUNX2基因的mRNA表达均上调。添加相同浓度PHT时,共培养组较单独培养组中rBMSCs的ICAM-1、KDR和RUNX2基因的mRNA表达量增高,而VCAM-1基因的mRNA表达量降低。结论:PHT可能促进大鼠骨髓间充质干细胞向血管内皮细胞分化。Objective： To investigate the effects of PHT on the differentiation of rat bone marrow mesenchymal stem cells（ BMSCs）into vascular endothelial cells（ VECs）. Methods： rBMSCs were cultured in indirect coculture system with VECs and independent culture system in the presence of phenytoin at 0,20 and 40 μg / ml respectively. After 14 d culture the mRNA expression levels of the intercellular adhesion molecule-1（ ICAM-1）,vascular cell adhesion molecules-1（ VCAM-1）,vascular endothelial growth factor receptor-2（ KDR） and Runt-related transcription factor 2（ RUNX2） were detected by real-time PCR. Results： The expression levels of ICAM-1,KDR and RUNX2 were upregulated in r BMSCs by PHT treatment. The expression levels of ICAM-1,KDR and RUNX2 in the co-culture groups were higher than those in the independent culture groups of rBMSCs treated by PHT at the same concentration. The expression level of VCAM-1 in co-culture groups was lower than that in the independent culture groups. Conclusion： PHT may promote r BMSCs differentiation into r VECs in the co-culture system.

关 键 词：大鼠骨髓间充质干细胞(rBMSCs) 血管内皮细胞(rVECs) 间接共培养 血管新生 苯妥英钠(PHT) 

分 类 号：R781[医药卫生—口腔医学]