柠檬精油、柠檬烯、茶多酚对变形链球菌表面疏水性及黏附的影响  

The effects of lemon essential oil,limonene and tea polyphenols on cell surface hydrophobicity and adherence of Streptococcus mutans

作　　者：张晨[1] 刘雅丽[1] 陈思佳[1] 张向宇[1] 

机构地区：[1]天津医科大学口腔医学院,300070

出　　处：《实用口腔医学杂志》2016年第4期471-474,共4页Journal of Practical Stomatology

基　　金：天津市应用基础及前沿技术研究计划(编号:10JCYBJCI3600);天津市高等学校科技发展基金(编号:20100133)

摘　　要：目的:研究天然植物成分柠檬精油(LEO)、柠檬烯(LIM)及茶多酚(TP)对变形链球菌(S.mutans)细菌表面疏水性及黏附能力的影响。方法:取最小抑菌浓度(MIC)以下浓度作为实验浓度;采用微生物黏着碳氢化合物法(MATH),测定S.mutans表面疏水性;采用96孔板结晶紫染色法,评价S.mutans的黏附。结果:LEO、LIM、TP在低于最小抑菌浓度(MIC)时,对S.mutans表面疏水性及粘附具有抑制作用;在一定范围内其抑制作用随浓度增大而逐渐增加(P<0.05);1/2 MIC和1/20MIC时LEO抑制表面疏水性的作用强于LIM和TP(P<0.05)。结论:LEO具有防龋应用前景。Objective： To explore the effects of the natural plant ingredients lemon essential oil（ LEO）,limonene（ LIM） and tea polyphenols（ TP） on the cell surface hydrophobicity and adherence of Streptococcus mutans（ S. mutans）. Methods： S. mutans were treated by sub-minimal inhibitory concentration（ MIC） levels of LEO,LIM and TP respectively. Adsorption to hexadecane was used to measure the hydrophobic interaction of S. mutans. A classical 96-cell microtitre plate production assay using crystal violet staining was employed to visualize the adherence of S. mutans to hard tissue surface. Results： LEO,LIM and TP at sub-MIC levels could inhibit the cell surface hydrophobicity and adherence of S. mutans in a dose-dependent manner（ P〈0. 05）. At 1 /2 MIC and 1 /20 MIC,the inhibitary effect of LEO was stronger than that of LIM and TP（ P〈0. 05）. Conclusion： LEO may possess anticariogenic potential.

关 键 词：柠檬精油(LEO) 变形链球菌(S.mutans) 细胞表面疏水性 黏附 

分 类 号：R780.2[医药卫生—口腔医学]