促红细胞生成素对人牙髓细胞增殖和成骨分化的影响  

The effects of erythropoietin on the proliferation and osteogenic differentiation of human dental pulp cells

作　　者：韩伟 

机构地区：[1]湖北省直属机关医院口腔科,武汉430071

出　　处：《实用口腔医学杂志》2016年第4期485-489,共5页Journal of Practical Stomatology

摘　　要：目的:探讨促红细胞生成素(EPO)对体外培养的人牙髓细胞(h DPCs)增殖和成骨分化的影响。方法:体外培养鉴定人牙髓细胞。使用EPO对在成骨诱导培养基中培养的牙髓细胞进行刺激,CCK-8检测EPO对牙髓细胞增殖的影响;20 U/ml EPO培养hDPCs 7 d和14 d,采用碱性磷酸酶活性(ALP)检测和茜素红染色观察EPO对牙髓细胞矿化的影响;利用Real-time PCR检测加入EPO后牙髓细胞成牙本质向分化相关基因的表达情况。结果:EPO以时间和剂量依赖性方式促进牙髓细胞的增殖;20 U/ml的EPO后作用,牙髓细胞碱性磷酸酶活性显著提高(P<0.05),钙结节形成明显增多;成牙本质向分化相关基因DSPP、OCN、OSTERIX、RUNX2的表达明显上调(P<0.05)。结论:EPO能促进人牙髓细胞的增殖和分化。Objective： To evaluate the effects of erythropoietin（ EPO） on the proliferation and osteogenic differentiation of human dental pulp cells（ hDPCs） in vitro. Methods： Isolated h DPCs were cultured and identified. The cells were treated by EPO and the proliferation of the cells was determined by CCK-8 assay. After incubation with EPO at 20 U / ml in osteogenic induction medium for 7and 14 days,the mineralization of h DPCs was observed by alkaline phosphatase（ ALP） activity assay and alizarin red staining. Realtime PCR was utilized to detect the expression of odontogenesis-related genes. Results： EPO enhanced the proliferation of hDPCs in a time-and dose-dependent manner. After treatment with EPO,ALP activity and the minerialized nodes of the cells increased（ P〈0. 05）; the expression levels of odontogenesis-related genes DSPP,OCN,OSTERIX and RUNX2 were upregulated（ P〈0. 05）. Conclusion： EPO can promote proliferation and differentiation of human dental pulp cells

关 键 词：促红细胞生成素 牙髓细胞 增殖 分化 

分 类 号：R781[医药卫生—口腔医学]