臭氧水溶液对牙周可疑致病菌作用的研究  

The bactericidal effect of ozonated water on the putative periodontopathic bacteria

作　　者：刘媛[1] 林川[1] 丁一[2] 

机构地区：[1]攀枝花市中心医院口腔科,617067 [2]四川大学华西口腔医院牙周科

出　　处：《实用口腔医学杂志》2016年第4期547-551,共5页Journal of Practical Stomatology

摘　　要：目的:评价臭氧水溶液对常见牙周可疑致病菌的杀菌效果。方法:采用细菌悬液定量实验法,测定不同浓度的臭氧水溶液对牙龈卟啉单胞菌(P.g)ATCC33277、伴放线菌嗜血菌(H.a)ATCC29522、具核梭杆菌(F.n)ATCC10957和牙龈卟啉单胞菌临床分离株作用30、60、90、120 s的杀菌率,阳性对照组采用不同浓度的过氧化氢溶液,阴性对照组采用蒸馏水。实验样本在厌氧培养72 h后进行菌落计数并计算杀菌率。结果:臭氧水溶液对几种牙周可疑致病菌均有杀灭效果,且杀菌率随浓度的增加而提高(P<0.01)。对P.g标准株和临床分离株的杀菌率差异无统计学意义(P>0.05)。线性回归分析臭氧水溶液对3种细菌杀灭作用的浓度影响因素β值均大于0.95,时间影响因素β值均小于0.11。结论:臭氧水溶液对牙龈卟啉单胞菌、伴放线菌嗜血菌和具核梭杆菌具有浓度依赖性杀灭效果。Objective： To evaluate the antimicrobial effect of ozonated water on the putative periodontopathic bacteria. Methods：Pophyromonas gingivalis（ P. g） ATCC33277,Haemophilus actinomycetemcomitans（ H. a） ATCC29522,Fusobacterium nucleatum（ F. n） ATCC10957 and clinically seperated strain of P. g（ C- P. g） were treated by ozonated water with ozone concentration（ mg/L）of 0. 03,0. 06 and 0. 12 for 30,60,90 and 120 s respectively. The bactericidal effect was tested by bactericidal assay. H_2O_2 was used as the positive control and distilled water as the negative control. Results： The antimicrobial rate of ozonated water agaist the bacteria increased with the ozone concentration increase. There was no statistic diffrence of the effect on P. g and C- P. g（ P〈0. 05）. Linear regression analysis showed that the β values of the concentration factor were over 0. 95,that of the time factor under 0. 11. Conclusion： The ozonated water has dose-dependent bactericidal effect on P. g,H. a and F. n.

关 键 词：臭氧 牙周可疑致病菌 杀菌作用 

分 类 号：R781.4[医药卫生—口腔医学]