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作 者:刘会云[1] 刘畅[1] 王坤杨 杜丽璞[1] 王轲[1] 佘茂云[2] 叶兴国[1]
机构地区:[1]中国农业科学院作物科学研究所/国家基因资源与遗传改良重大科学工程,北京100081 [2]安徽省农业科学院作物研究所,合肥230031
出 处:《植物遗传资源学报》2016年第4期701-709,共9页Journal of Plant Genetic Resources
基 金:国家自然科学基金(31371621);安徽省农业科学院科技创新团队项目(13C0202);安徽省种子工程项目(14D0202)
摘 要:高分子量谷蛋白亚基(HMW-GS,high molecular weight glutenin subunits)是小麦子粒贮藏蛋白的重要组成成分,其组成、搭配、表达水平及含量决定面团弹性和面包加工品质。本文主要介绍了小麦HMW-GS编码基因的克隆、分子特征、分子标记开发及其在小麦育种中的应用,并综述了不同HMW-GS与面粉加工品质之间的关系,以及HMW-GS基因遗传转化、微量配粉和突变体培育等方面的研究进展,分析了目前研究中存在的主要问题,认为通过分子标记辅助选择和转基因技术聚合优质亚基,培育优质面包小麦品种和明确各个HMW-GS基因的品质效应是今后的研究重点。High molecular weight glutenin subunits( HMW-GS) are the most important components in the storage protein of wheat grains. Their compositions,expressions and contents determine the dough elasticity and baking-quality. This article summarized the progress of cloning,molecular characterization and functional studies of HMW-GS as well as their marker development and application in wheat breeding. Update status of different HMWGS effects on flour processing quality,genetic transformation of several HMW-GS genes,small-scale mixograph analysis by adding prokaryotic expressed HMW-GS,and mutant developing on HMW-GS loci,were also reviewed.Besides,crucial issues in current studies on wheat HMW-GS were discussed. It is suggested that the key point on HMW-GS in the future will be to pyramid some desirable HMW-GS genes by marker assisted selection and transgenic strategies for the development of wheat varieties with good bread-making quality and the functional dissection of each HMW-GS gene accurately.
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