细胞分裂素6-BA对三裂叶野葛毛状根生长和抗氧化酶活性的影响  

Effect of 6-Benzylaminopurine on Growth and Antioxidase Activities in Hairy Root Cultures of Pueraria phaseoloides

作　　者：何含杰[1] 黄小西[1] 张党权[1] 邓华凤[2] 

机构地区：[1]中南林业科技大学林业生物技术湖南省重点实验室/生物发育工程及新产品研发协同创新中心,湖南长沙410018 [2]杂交水稻国家重点实验室湖南杂交水稻研究中心,湖南长沙410125

出　　处：《北方园艺》2016年第14期156-159,共4页Northern Horticulture

基　　金：湖南省自然科学基金资助项目(2015JJ3178);长沙市科技攻关资金专项资助项目(K1406011-21);中南林业科技大学青年基金资助项目(QJ2012006B)

摘　　要：以三裂叶野葛毛状根为试材,研究了不同浓度6-BA对三裂叶野葛毛状根生长、异黄酮化合物及可溶性蛋白质含量和抗氧化酶活性的影响。结果表明:6-BA抑制三裂叶野葛毛状根的生长,降低其鲜样质量,且抑制作用与6-BA浓度成正比例关系,同时能提高毛状根培养物中异黄酮化合物的含量;但降低其毛状根培养物中可溶性蛋白质含量和超氧化物歧化酶(superoxide dismutase,SOD)活性水平,提高过氧化物酶(peroxidase,POD)活性水平,这表明6-BA可能通过调节三裂叶野葛毛状根的抗氧化酶活性进而调控其生长和异黄酮化合物的积累。Using the hairy roots of Pueraria phaseoloides cultured in solid mediums as an experiment material,the effect of 6-Benzylaminopurine （6-BA） on growth, contents of isoflavone compounds and soluble protein and antioxidase activities was studied. The results showed that plant growth regulator 6-BA inhibited the growth of P. phaseoloides hairy roots, reduced the biomass of fresh weight of hairy roots compared with control, and the inhibition was in proportion to the concentration of 6-BA. Furthermore, 6-BA promoted synthesis and accumulation of isoflavone compounds in hairy root cultures. Compared with control, 6-BA decreased the contents of soluble protein and activities of superoxide dismutase in hairy root cultures, hut enhanced the activities of peroxidase. These results indicated that plant growth regulator 6-BA regulated the growth and accumulation of isoflavone compounds through regulating antioxidase activities of P. phaseoloides hairy roots.

关 键 词：三裂叶野葛 毛状根 6-BA 异黄酮化合物 可溶性蛋白质 抗氧化酶 

分 类 号：S565.4[农业科学—作物学]