子宫内膜不典型增生患者内膜组织中错配修复蛋白的表达情况  

Expression of mismatch repair protein in endometrial hyperplasia tissues of patients with atypical hyperplasia of endometrium

作　　者：刘彩虹 陈志强[2] 

机构地区：[1]河北省承德市妇幼保健院病理科,河北承德067000 [2]河北省承德市中心医院泌尿外科,河北承德067000

出　　处：《中国医药导报》2016年第20期80-83,共4页China Medical Herald

基　　金：河北省医学科学研究重点课题计划项目(20160289)

摘　　要：目的 探讨子宫内膜不典型增生患者子宫内膜组织中错配修复（MMR）蛋白的表达情况及微卫星序列的不稳定性（MSI）。方法 选取承德市中心医院2005年10月~2015年6月存档的200例患者的子宫内膜增生组织为研究对象,按增生类型分为单纯性增生组（n=50）、复杂性增生组（n=50）、单纯性非典型增生组（n=50）及复杂性非典型增生组（n=50）四组,采用免疫组织化学法检测四组内膜组织中MMR蛋白（MLH1、MSH2、PMS2、MSH6）的表达情况,比较四组的MMR失表达率,观察非典型增生患者的MSI[低度微卫星不稳定（MSI-L）、高度微卫星不稳定（MSI-H）]和MSS表型与年龄的关系。结果 复杂性非典型增生组的MLH1、MSH2、PMS2及MSH6的失表达率高于其他三组,且单纯性非典型增生组、复杂性增生组、单纯性增生组的MMR失表达率逐渐降低,四组患者的MLH1、MSH2、PMS2失表达率比较,差异均有统计学意义（P〈0.05）,但四组MSH6失表达率比较差异无统计学意义（P〉0.05）;MSI-H、MSI-L和MSS表型在单纯性增生组、复杂性增生组、单纯性非典型增生组及复杂性非典型增生组中的表型比较,差异均有统计学意义（P〈0.05）;在单纯性非典型增生组和复杂性非典型增生组中,MSI-H、MSI-L及MSS表型在年龄≤50岁和〉50岁患者间的差异均有统计学意义（P〈0.05）。结论 MMR蛋白的失表达及微卫星不稳定性可能是子宫内膜不典型增生患者的重要分子事件。Objective To explore the expression of mismatch repair（MMR） gene proteins and microsatellite instability（MSI） in atypical endometrial hyperplasia. Methods 200 endometrial hyperplasia tissues from October 2005 to June2015 in the Central Hospital of Chengde City were selected, and they were divided into the simple hyperplasia group（50 cases）, complexity hyperplasia group（50 cases）, simple atypical hyperplasia group（50 cases） and complexity atypical hyperplasia group（50 cases） according to hyperplasia type. Immunohistochemical method was used to detect the expressions of DNA mismatch repair proteins（MLH1, MSH2, PMS2 and MSH6） in four groups; the non-expression rate of MMR were compared between four groups; the relationships between MSI（MSI-L, MSI-H） and age of atypical hyperplasia group was also observed. Results The non-expression rate of MLH 1, MSH2, PMS2 and MSH6 were the highest in the complexity atypical hyperplasia group, and non-expression rate of MLH1, MSH2, PMS2 in the simple atypical hyperplasia group, complexity hyperplasia group and simple hyperplasia group were decreased progressively, the differences were statistically significant（P 0.05）; while there was no statistically significant difference in the non-expression rate of MSH6 in the four groups（P 0.05）. There were statistically significant differences in phenotypes of MSIH, MSI-L and MSS in the four groups（P 0.05）. There were statistical significance in the expression of MSI-H, MSIL and MSS between patients who age ≤ 50 years and 50 years in the atypical hyperplasia group（P 0.05）. Conclusion MSI and the negative expression of MMR proteins may be one of the important molecular events of patients with atypical endometrial hyperplasia.

关 键 词：子宫内膜不典型增生 错配修复蛋白 微卫星不稳定性 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]