电针预处理对大鼠脑缺血再灌注损伤后炎性因子及细胞凋亡的影响  

Effects of Electro-acupuncture Pretreatment on Interleukin-1β, Interleukin-6 and Apoptosis in Rats after Cerebral Ischemia-reperfusion Injury

作　　者：朱路文[1] 叶涛[2] 姜云飞[2] 吴孝军[2] 李宏玉[2] 宋名扬 唐强[1] 

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨市150001 [2]黑龙江中医药大学,黑龙江哈尔滨市150040

出　　处：《中国康复理论与实践》2016年第7期765-768,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：哈尔滨市科技创新人才专项基金项目(青年后备人)(No.2014RFQGJ150);黑龙江省自然科学基金项目(No.QC2015103);国家自然科学基金项目(No.81503666)

摘　　要：目的探讨电针预处理对大鼠脑缺血再灌注损伤后白细胞介素(IL)-1β、IL-6含量及细胞凋亡的影响。方法 36只清洁级雄性Sprague-Dawley大鼠按随机数字表法分为假手术组、模型组和电针预处理组,每组12只。大脑中动脉阻塞(MCAO)建立脑缺血2 h再灌注模型。再灌注后24 h,酶联免疫吸附法检测血清、脑组织IL-1β、IL-6的含量,TUNEL染色检测缺血半暗区神经细胞凋亡。结果与模型组比较,电针预处理组大鼠血清、脑组织IL-1β、IL-6的含量明显下降(P<0.01),TUNEL阳性细胞数显著下降(P<0.001)。结论电针预处理降低炎症反应,减少细胞凋亡。Objective To investigate the effect of electro-acupuncture pretreatment on content of interleukin（IL）-1β and IL-6 in serum and ischemic penumbra, and apoptosis in ischemic penumbra in rats after cerebral ischemia- reperfusion injury. Methods Thirty- six male Sprague-Dawley rats were randomly divided into sham group（n=12）, model group（n=12）, and electro-acupuncture pretreatment group（n=12）. The middle cerebral arteries were occluded for 120 minutes and reperfused. Twenty-four hours after reperfusion, the level of IL-1β and IL-6 in serum and brain tissue was detected with enzyme-linked immunosorbent, and apoptosis of ischemic penumbra was detected with TUNEL. Results The content of IL-1β and IL-6 in the serum and brain tissue, and the number of TUNEL-positive cells decreased in the electro-acupuncture pretreatment group compared with that in the model group（P〈0.01）. Conclusion Electro-acupuncture pretreatment may inhibit inflammatory response and apoptosis in cerebral ischemia-reperfusion injury.

关 键 词：脑缺血再灌注 电针 预处理 白细胞介素-1Β 白细胞介素-6 细胞凋亡 炎症反应 大鼠 

分 类 号：R743.32[医药卫生—神经病学与精神病学]