人结肠癌原位移植瘤裸小鼠模型的活体成像观察  

Dynamic Observation on In vivo Biofluorescent Imaging of Orthotopic Transplant Model of Human Colon Cancer in Nude Mice

作　　者：赵永江 朱淼鑫[2] 袁立新 孙磊[2] 耿沁[2] 李静[2] 姚明[2] 闫明霞[2] 

机构地区：[1]上海市同仁医院影像介入科,上海200023 [2]上海市肿瘤研究所,上海200032

出　　处：《实验动物与比较医学》2016年第3期174-179,共6页Laboratory Animal and Comparative Medicine

基　　金：上海市科学技术委员会“创新行动计划”项目（13140900502;13140902102）

摘　　要：目的 建立人结肠癌原位移植瘤裸小鼠模型,探讨小动物活体成像系统在结肠癌原位移植瘤模型中的应用.方法 应用慢病毒转染的方法建立同时表达绿色荧光蛋白及荧光素酶（GFP/Luc）的人结肠癌细胞HCT-116,分别采用组织决法及培养细胞法建立裸小鼠结肠癌原位移植瘤模型,通过小动物活体成像系统动态观察肿瘤在肠原位的生长及转移情况.结果 成功建立了稳定表达GFP/Luc的人结肠癌细胞,并应用该细胞成功建立了结肠癌原位移植瘤动物模型.经连续7周动态观察表明,随着肿瘤移植时间的延长,腹部皮下触诊显示肿瘤逐渐增大,小动物活体成像显示荧光素表达面积和强度也逐渐增大.结论 小动物活体成像系统能够客观评价结肠癌的原位生长及转移情况,为体内深部肿瘤的观察和研究提供了有效的参考依据.Objective To establish an orthotopic transplant nude mice model of human colon cancer and discuss the application of in vivo biofluorescent imaging system in animal model. Methods Human colon cancer cells were tranfected with green fluorescent protein/Luciferase gene by lentiviral vector, they were orthotopically transplanted into the cecum of nude mice by using tumor tissues or cell culture method. The tumor growth and metastasis were dynamically observed by using in vivo biofluorescent imaging system. Results The human colon cancer cell line and orthotopic transplant nude mice model were successfully established, which could display stable high-level GFP/Luc expression in vitro and in vivo. In vivo biofluorescent images showed that the fluorescent areas and intensities were increased gradually with the increase of tumor volume in nude mice during 7 weeks of dynamic observation. Conclusions In vivo biofluorescent imaging system was fitted for evaluation and quantitation of the tumor growth and metastasis of colon cancer. It would provide effective reference for the observation and study of in vivo deep tumors.

关 键 词：人结肠癌 原位移植 活体成像系统 慢病毒载体 

分 类 号：R735.35[医药卫生—肿瘤] R-332[医药卫生—临床医学]