面神经夹闭建立大鼠面瘫模型  

Establishing Facial Paralysis Model in Rats by Clipped Facial Nerve

作　　者：李瑶[1] 高小莉[2] 陈健康[3] 王四旺[2] 谢艳华[2] 

机构地区：[1]陕西中医药大学药学院,咸阳712046 [2]第四军医大学药学系天然药物学教研室,西安710032 [3]第四军医大学教学实验中心,西安710032

出　　处：《实验动物与比较医学》2016年第3期190-194,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的 建立操作简便、发病率高、死亡率低的大鼠面瘫模型制作方法.方法 SD大鼠50只,雌雄各半,随机分为5组,每组10只,分别用蚊式钳闭合三齿夹闭大鼠右侧耳后面神经主干6 min、8 min、10 min、12 min、14 min,记录各组大鼠面神经复合动作电位（CMAP）波幅变化情况,观察大鼠的一般行为学指标.结果 造模6 min、8 min与造模之前比较,面神经CMAP波幅无差异,造模10 min、12 min、14 min与造模之前相比较,大鼠面神经CMAP波幅明显减小并趋于0;行为学评分结果表明,造模6 min、8 min大鼠为不完全面瘫,造模10 min、12 min、14 min大鼠完全面瘫,造模12 min、14 min大鼠与造模10 min相比无显著差异.结论 用蚊式钳闭合三齿夹闭面神经主干10 min,面神经CMAP下降波幅和行为学评分均表明大鼠完全面瘫,故该方法可作为大鼠面瘫模型建立的优选方法.Objective To establish a high morbidity and low mortality of animal model for facial paralysis in rats, which is easy to operate. Methods Fifty SD rats with half male and female were randomly divided into five groups, each group with 10. The facial nerve trunk of rats were clipped with mosquito forceps closing three tooth for 6 rain, 8 rain, 10 min, 12 min and 14 min. The compound muscle action potential （CMAP） amplitude of facial nerve trunk was measured by biological function experiment system, and the general behavior indices of all the rats were observed next day after injury. Results The CMAP amplitude of rats after facial nerve trunk clipped for 6 rain and 8 rain respectively were the same as none clipped rats, but it decreased obviously and tended to zero after clipped for 10 min, 12 min and 14 min. In addition, ethology score results showed that the models of 6 min, 8 min rats performed incomplete paralysis symptoms, the models of 10 rain,12 rain, 14 min rats were facial paraly- sis completely and the models of 14 min and 12 min rats had no obvious difference compared with the model of 10 min. Conclusion The decreased CMAP amplitude of facial nerve trunk and ethology score results showed that clipping the facial nerve trunk with the mosquito forceps closing three tooth for 10 min was the best way to establish rats model of facial paralysis.

关 键 词：大鼠 面瘫模型 面神经 复合动作电位 

分 类 号：R745.12[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]