雌激素灌胃法制备豚鼠子宫肌瘤模型  

Intragastric Estrogen Administration Induced Guinea Pig Model of Uterine Leiomyoma

作　　者：张玥晗 文静[1] 朱海桃 赵光宗[2] 李晓愚[3] 

机构地区：[1]重庆医科大学2012级临床一系,重庆401331 [2]重庆医科大学2013级临床二系,重庆401331 [3]重庆医科大学实验教学管理中心,重庆401331

出　　处：《实验动物与比较医学》2016年第3期195-199,共5页Laboratory Animal and Comparative Medicine

基　　金：重庆市大学生创新创业计划项目201410631021;重庆医科大学大学生科学研究与创新实验项目201423

摘　　要：目的 用雌激素灌胃法制备豚鼠子宫肌瘤模型,探讨不同剂量及给药周期对子宫肌瘤形成的影响.方法 性成熟雌性豚鼠分为短周期组和长周期组,分别每周2次灌胃戊酸雌二醇（补佳乐）0.1 mg/100g体质量6周和8周.空白对照组灌胃相同体积生理盐水.灌胃结束后,ELISA法测定血清中雌二醇及孕酮水平;计算肌瘤形成率、子宫脏器系数;子宫组织制作石蜡切片,检测子宫肌瘤模型是否成功建立.结果 短周期模型组成瘤率30％,长周期模型组成瘤率40％,总体成瘤率35％.血清学检测显示模型组动物血清雌激素水平明显增高,孕激素水平下降;病理组织学检测证实造模成功.结论 雌激素灌胃法是一种简单有效的豚鼠子宫肌瘤模型造模方法.Objective To establish the guinea pig model of uterine leiomyoma by intragastric administration of estrogen and to discuss the effect of different cycles of administration on formation of uterine leiomyoma. Method Mature female guinea pigs were divided into short term group and long term group, and intragastric administrated with estradiol valerate at 0.1 mg/100 g body weight twice a week for 6 weeks and 8 weeks respectively. The control was intragastric administrated with normal saline with same volume. After 6 or 8 weeks, the guinea pigs were sacrificed. The serum levels of estrogen and progesterone were measured by ELISA and the leiomyoma forming rate was measured. Histological changes were compared between treatment group and control group with HE staining. Results The leiomyoma forming rate in short term group was 30% and long term group was 40%. The average total rate was 35%. The serum level of estrogen in model animals was increased significantly and the progesterone decreased in long term group. Histopathological examination confirmed that the guinea pig model of uterine leiomyoma were established successfully. Conclusions Intragastric estrogen administration was a simple and effective method for establishing uterine leiomyoma guinea pigs model.

关 键 词：子宫肌瘤 豚鼠 雌激素 灌胃法 

分 类 号：R737.33[医药卫生—肿瘤] R-332[医药卫生—临床医学]