植物发酵液对肠易激综合征患者肠道菌群的调节  

Effects of plant fermentation extract on intestinal microbiota in patients with irritable bowel syndrome

作　　者：杨莉丽[1] 吴彬彬[2] 梁岩[2] 

机构地区：[1]北京大学深圳医院消化内科,广东省深圳市518036 [2]中国科学院深圳先进技术研究院食品安全与环境技术研究室,广东省深圳市518055

出　　处：《世界华人消化杂志》2016年第19期2982-2987,共6页World Chinese Journal of Digestology

基　　金：深圳市科技计划基金资助项目;Nos.JCYJ20140415162542973;CXZZ20150422152108120~~

摘　　要：目的:通过高通量测序方法研究植物发酵液(plant fermented extract,PFE)对肠易激综合征(irritable bowel syndrome,IBS)患者肠道菌群的影响.方法:分别收集PFE作用0 d和30 d的人体粪便样本,采用Illumima系统MiSeq平台对PFE作用前后的样本进行16SrRNA测序,分析肠道菌群的结构和丰度.结果:PFE作用后,IBS患者的肠道菌群多样性无显著变化.在门水平上,硬壁菌门(Firmicutes)丰度显著降低(P<0.01),拟杆菌门(Bacteroidete)和变形菌门(Proteobacterias)的丰度以及Bacteroidetes/Firmicutes的比值增加.在最佳分类水平(科水平)上,P F E作用后的粪便肠道菌群中检测到弯曲菌科(Campylobacteraceae)、蟑螂杆状体科(Cryomorphaceae)、丹毒丝菌科(Erysipelotrichaceae)和纤维杆菌科(Fibrobacteraceae),且丰度变化具有显著差异(P<0.05).结论:PFE能够改善IBS患者肠道菌群丰度,调节肠道菌群,可能在IBS疾病的诊断和治疗中具有重要作用.AIM：To investigate the effect of plant fermentation extracts（PFE） on intestinal microbiota in patients with irritable bowel syndrome（IBS）,using high-throughput sequencing.METHODS：The feces were collected from IBS patients before（day 0） and 30 days（day30） after consumption of PFE,respectively.The community structure and abundance of the intestinal microbiota were examined via analyzing 16 S rRNA high throughput sequencing using an Illumima System.RESULTS：The overall diversity of the intestinal microbiota had no significant change on day30.At the phylum level,the abundance of Firmicutes was significantly decreased（P〈0.05）,while that of Bacteroidetes was significantly increased（P〈0.05）.The ratio of Bacteroidetes/Firmicutes was greatly elevated.At the family level,Campylobacteraceae,Cryomorphaceae,Erysipelotrichaceae and Fibrobacteraceae were detected after the PFE consumption,and their abundance all significantly increased（P〈0.05）.CONCLUSION：PFE improves the abundance of intestinal microbiota in IBS patients,which may be utilized as a type of dietary intervention of IBS.

关 键 词：植物发酵液 肠易激综合征 肠道菌群高通量测序 16SrRNA 

分 类 号：R259[医药卫生—中西医结合]