中药酚酸类成分的纳滤分离行为对比研究  

Comparative Study of Separation on Phenolic Acids Components by Nanofiltration Process

作　　者：瞿其扬 方诗琦 彭国平[1,2] 李红阳[1] 时浩[1] 顾佳美 李存玉[1,2] 

机构地区：[1]南京中医药大学药学院,南京210023 [2]江苏省中药资源产业化过程协同创新中心,南京210023

出　　处：《中国药房》2016年第21期2965-2968,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81503258)

摘　　要：目的：探讨不同孔径聚酰胺纳滤膜对中药酚酸类成分纳滤分离的影响及其纳滤浓缩的适用性。方法：选择常用药材丹参、金银花及制剂中间体茶多酚提取物,以其中含有的酚酸类成分为研究对象,以制剂生产中常用的水提液作为纳滤原液,经不同孔径聚酰胺纳滤膜处理后,通过高效液相色谱法分析药液中各成分纳滤前后的含量变化。结果：丹参素、原儿茶醛、咖啡酸等均能较好地透过3种孔径的纳滤膜;迷迭香酸、绿原酸、表没食子儿茶素没食子酸酯、新绿原酸、表儿茶素没食子酸酯等成分随膜孔径变小,截留率有不同程度提升;而丹酚酸B除了600～800 Da膜有少量截留外,在低分子质量膜中几乎全被截留。结论：分子质量对中药酚酸类成分的纳滤分离行为有重要影响;纳滤浓缩对中药酚酸类成分提取有较好的应用前景。OBJECTIVE：To investigate the effect of polyamide on the separation on phenolic acids components by nanofiltration process. METHODS：Phenolic acids components were selected as targets,extracted from the Salvia miltiorrhiza,Lonicera japonica or intermediate preparations（tea polyphenols）with water,and filtered with membranes with different pore sizes. The content changes of components were analyzed by HPLC. RESULTS：Danshensu,protocatechuic aldehyde and caffeic acid could better transmit nanofiltration membranes with 3 different pore sizes;with the decrease of pore size,the rejection rates of neochlorogenic acid,chlorogenic acid,rosmarinci acid,ECG and EGCG grew in different levels. But salvianolic acid B was almost retained by the membrane with low molecular weight except 600-800 Da. CONCLUSIONS：Molecular weight importantly influenced nanofiltration separation on phenolic acid components;nanofiltration has a good prospect in enrichment of phenolic acids components.

关 键 词：纳滤膜 酚酸 截留率 分子质量 

分 类 号：R282.71[医药卫生—中药学]