藤茶多酚分离纯化及其对口腔变形链球菌抑制作用研究  

Separation and purification of polyphenols from Ampelopsis grossedentata and its inhibition effect on oral mutating streptococci

作　　者：蒋旭东[1] 杨卫豪[2] 周传检[2] 卢凯玲[2] 林翠梧[3,4] 

机构地区：[1]广西科技大学医学院,广西柳州545005 [2]柳州两面针股份有限公司,广西柳州545006 [3]广西大学化学化工学院,广西南宁530004 [4]广西高校应用化学技术与资源开发重点实验室,广西南宁530004

出　　处：《日用化学工业》2016年第7期414-418,426,共6页China Surfactant Detergent & Cosmetics

基　　金：柳州两面针股份有限公司博士后基金资助项目(2013)

摘　　要：采用Folin-Ciocaileu比色法测定藤茶多酚质量浓度,计算吸附率和洗脱率,比较5种不同性质大孔树脂对藤茶多酚静态吸附和解吸的影响,优选出HPD-100型树脂。进一步通过单因素试验得到藤茶多酚分离纯化的优选工艺条件为:上样液藤茶多酚粗提物质量浓度2.4 g/L、流速2.0 BV/h、体积6.5 BV;洗脱剂乙醇体积分数60%、流速3 BV/h、体积7.5 BV。在此条件下,经大孔树脂处理后藤茶多酚质量分数由29.9%提高到69.8%。抑菌试验表明,经大孔树脂处理后的藤茶提取物具有显著抑制口腔变形链球菌生长的作用,其最低抑菌浓度为3.13 g/L。Via the determination of polyphenol mass concentration by Folin- Ciocaileu colorimetry,calculation of the adsorption and elution rate,as well as comparison of effect of five kinds of macroporous resin on static adsorption and desorption rates of polyphenols from Ampelopsis grossedentata,HPD- 100 resin was selected to conduct the optimization of purification technology by single factor tests. Results showed that the optimum purification conditions are： extract of polyphenol mass concentration of feed sample,2. 4 g / L of 6. 5 BV,flow rate,2. 0 BV / h; elution with 7. 5 BV of 60% ethanol at a flow rate of 3 BV / h. The mass fraction of polyphenols can be enhanced from 29. 9% to 69. 8%. The results showed that the inhibition activity of polyphenols from Ampelopsis grossedentata on growth of oral mutating streptococci is very obvious and the MIC value achieves 3. 13 g / L.

关 键 词：口腔护理 藤茶多酚 纯化 变形链球菌 

分 类 号：TS272[农业科学—茶叶生产加工] R780.2[轻工技术与工程—农产品加工及贮藏工程]