实验室室间质评染色体检测水平效果分析  

Assessment of effect of laboratory external quality assessment program on cytogenetic testing

作　　者：陈希[1] 陈园园[1] 牟琴[1] 杨静[1] 范玲[1] 张大勇[1] 王治国 豆虎[3] 邹琳[1] 包黎明[1] 

机构地区：[1]重庆医科大学附属儿童医院临床分子医学中心/重庆市儿科学重点实验室/儿童发育疾病研究省部共建教育部重点实验室,重庆400014 [2]国家卫生计生委临床检验中心,北京100730 [3]重庆医科大学附属儿童医院检验科,重庆400014

出　　处：《重庆医学》2016年第21期2917-2919,共3页Chongqing medicine

基　　金：重庆市卫生局课题资助项目(2012-2-100)

摘　　要：目的分析参加美国病理学会（CAP）室间质评的能力比对检验（PT）后,染色体检测水平的变化。方法分析2011-2014参加CAP PT的结果,并根据CAP评审结果,得出该实验室细胞遗传学染色体质控测试的准确率,并通过分析该实验室参加CAP前后的染色体条带水平、异常检出率及染色体检测失败率,评估CAP室间质评项目对提高该实验室染色体检测水平的作用。结果 2011-2014年该实验室参加CAP PT测试10次,共分析病例59例,判断染色体核型正确率为100%,核型描述正确率为95.1%。该实验室临床病例染色体检测结果显示：外周血染色体的异常检出率为18.9%,骨髓染色体的异常检出率为25.9%,初诊白血病异常率为66.8%;外周血染色体及骨髓染色体检测的失败率分别为0.5%、5.0%;参加CAP后,外周血及骨髓染色体检测失败率均下降,染色体条带平均水平均提高,与参加CAP前比较差异均有统计学意义（P〈0.01）。结论参加高水平的室间质评项目可以提高细胞遗传学染色体检测的临床分析水平。Objective To analyze the change of chromosome test level after participating in the inter-laboratory proficiency test（PT）program of the College of American Pathologists（CAP）.Methods The results for participating in CAP PT during2011-2014 were analyzed,the accuracy of the cytogenetic chromosome quality control test in this laboratory was obtained according to the CAP evaluation results,and the effect of CAP external quality assessment item on increasing the chromosome detection level in this laboratory was evaluated by analyzing the chromosome band levels before and after participating in CAP.Results This laboratory participated in CAP PT test for 10 times during 2011-2014,a total of 59 cases were analyzed,the accuracy rate for judging chromosome karyotype was 100%,the karyotype description accuracy rate was 95.1%.The chromosome test results of clinical cases in this laboratory displayed that peripheral blood chromosome abnormal detection rate was 18.9% and bone marrow chromosome abnormal detection rate was 25.9%,the abnormal rate of newly diagnosed leukemia was 66.8%;the detection failure rates of peripheral blood chromosome and bone marrow chromosome were 0.5% and 5.0% respectively;the detection failure rates of peripheral blood chromosome and bone marrow chromosome after participating in CAP were decreased,the chromosome band average level was improved,showing statistical difference compared with those before participating in CAP（P〈0.01）.Conclusion Participating in high quality external laboratory assessment item can increase the clinical analytic level of cytogenetic chromosome test.

关 键 词：细胞遗传学 室间质评 能力比对检验 

分 类 号：R440[医药卫生—诊断学]