酒精对肝脏蛋白酶体成熟的影响  

Effect of alcohol on proteasome assembly in hepatic tissue

作　　者：张莉[1] 阎明[2] 江堤[1] 孙贤久[1] 左海军[1] 刘美红[1] 

机构地区：[1]东莞东华医院消化内科,广东东莞523000 [2]山东大学齐鲁医院消化内科,山东济南250012

出　　处：《分子诊断与治疗杂志》2016年第4期268-271,共4页Journal of Molecular Diagnostics and Therapy

基　　金：山东省医药卫生科研重点项目(2007Hz056)

摘　　要：目的建立大鼠酒精性肝病模型,分析蛋白酶体成熟蛋白(proteasome maturation protein,POMP)的表达量,探讨酒精对肝脏蛋白酶体成熟的影响。方法利用灌胃的方法建立大鼠酒精性肝病模型,分批于6、8、10、12周处死大鼠。采用荧光定量PCR方法测定POMP m RNA表达量。结果荧光定量PCR显示蛋白酶体成熟蛋白POMP基因表达水平随摄酒增多呈逐渐升高趋势(P<0.05)。结论酒精摄入可影响蛋白酶体成熟,酒精导致的蛋白酶体成熟机制异常可能在蛋白酶体功能下降机制中起重要作用。Objective To evaluate the expression of proteasome maturation protein（POMP） in the rat models of alcoholic liver, and investigate the effect of alcohol on proteasome assembly. Methods Establish the rat models of alcoholic liver disease by gastric perfusion method, and kill the rats after 6, 8, 10, 12 weeks. The expressions of m RNA for POMP were detected by fluorogenic quantitative polymerase chain reaction. Results The expressions of m RNA for POMP increased gradually along with the increase of alcohol administration. Conclusion Alcohol administration resulted in the assembly of proteasome. Ethanol induced proteasome malfunction might be responsible for the obstacles of proteasome mature mechanism.

关 键 词：大鼠 酒精性肝病 蛋白酶体 蛋白酶体成熟蛋白 蛋白质氧化损伤 

分 类 号：R575[医药卫生—消化系统]