血浆MAL基因甲基化检测对于肺癌早期诊断的意义  

Plasma MAL gene methylation detection for early diagnosis of lung cancer

作　　者：黄大业[1] 张潍[1] 郭琪 

机构地区：[1]西安交通大学第二附属医院胸外科,陕西西安710004 [2]西安市胸科医院外科,陕西西安710004

出　　处：《现代肿瘤医学》2016年第13期2055-2058,共4页Journal of Modern Oncology

基　　金：陕西省科技攻关资助项目(编号:2012k13-02-30)

摘　　要：目的:研究肺癌患者、肺结核患者及健康人群T淋巴细胞相关蛋白(T-lymphocyte maturation-associated protein,MAL)基因启动子区的甲基化状态,进一步探讨血浆MAL基因甲基化检测对于肺癌早期诊断及鉴别肺癌与肺结核疾病的意义。方法:采用甲基化特异性PCR(methylation special PCR,MSP)方法,检测75例肺癌组织标本、癌旁组织标本和对应的血浆标本及58例肺结核组织标本及对应的血浆标本的MAL基因启动子区甲基化状态;同时检测30例正常人群的血浆标本MAL甲基化状态作为对照。结果:肺癌组织和癌旁组织中MAL基因甲基化发生率分别为78.7%(59/75)和2.7%(2/75),差别具有统计学意义(P<0.001);肺癌和肺结核组织中MAL基因甲基化发生率分别为78.7%(59/75)和3.4%(2/58),差别具有统计学意义(P<0.001);肺癌患者与肺结核患者对应的血浆中MAL基因甲基化发生率分别73.3%(55/75)和1.7%(1/58),差别也具有统计学意义(P<0.001)。正常健康人群的血浆标本中未发现MAL基因甲基化改变;肺癌组和肺结核组分别与健康人群组比较,肺癌组明显高于健康人群(P<0.001),而肺结核组患者与健康人群比较无明显差异(P=0.47)。组织标本和血浆标本的检出率具有一致性(P>0.05)。根据病理类型、TNM分期、性别、吸烟史等对肺癌患者进行分组,各组之间均无明显差异(P>0.05)。结论:MSP法检测血浆MAL基因甲基化状态,可以作为一种有潜力的肺癌早期诊断方法,对于鉴别肺癌和肺结核也有一定的帮助。Objective：To study T lymphocyte related protein（T -lymphocyte maturation -associated protein, MAL）gene promoter region methylation status of patients with lung cancer,tuberculosis and healthy people and ex-plore the vaue of plasma MAL gene methylation detection for early diagnosis of lung cancer and tuberculosis.Meth-ods：By methylation specific PCR（methylation special PCR,MSP）detection method,to detect 75 cases of lung cancer tissue samples,tissue adjacent to carcinoma specimens and the corresponding plasma specimens and 58 cases of tu-berculosis tissue specimens and the corresponding plasma specimen MAL gene promoter region methylation status.Al-so detecting serum specimens of 30 cases of normal crowd MAL methylation status in comparison.Results：In lung cancer tissues and in tissue adjacent to carcinoma MAL gene methylation incidences were 78.7%（59 /75）and 2.7%（2 /75）,the difference was statistically significant（P〈0.001）.MAL gene methylation in lung cancer and tuberculo-sis incidence was 78.7%（59 /75）and 3.4%（2 /58）（P〈0.001）.In the plasma of patients with lung cancer and pulmonary tuberculosis patients with corresponding MAL gene methylation rate 73.3%（55 /75）and 1.7%（1 /58） respectively（P〈0.001）.In normal plasma samples of healthy controls MAL gene methylation was not found.Lung cancer and tuberculosis group,respectively,compared with healthy group,lung cancer group was significantly higher than healthy people（P〈0.001）,and pulmonary tuberculosis patients compared with healthy people there was no ob-vious difference（P =0.47）.Tissue samples and plasma samples detection rate was consistent（P〉0.05）.For patho-logical type,TNMstaging,gender,smoking history,there were no obvious difference between groups（P〉0.05）.Con-clusion：MSP method to detect the plasma MAL gene methylation status,can be used as a kind of lung cancer early di-agnosis method for differentiating lung cancer and tuberculosis.

关 键 词：肺癌 肺结核 血浆DNA MAL 甲基化 诊断 

分 类 号：R734.2[医药卫生—肿瘤]