中央型和效应型记忆T细胞亚群在肝癌患者肿瘤浸润淋巴细胞中的分布  

Distribution of central and effector memory T cells subsets in tumor infiltrating lymphocytes of HCC patients

作　　者：骆必伟 谢勇[2] 袁晓东[3] 田佩凯[2] 欧希[2] 林泽伟[2] 刘晓平[2] 刘吉奎[2] 陈心春[4] 张明霞[4] 

机构地区：[1]深圳市人民医院肝胆外科,518020 [2]北京大学深圳医院肝胆腔镜外科,深圳518036 [3]安徽医科大学,合肥230032 [4]深圳市第三人民医院深圳市感染免疫重点实验室,518112

出　　处：《中华肝胆外科杂志》2016年第7期445-449,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：深圳市科技计划项目（JC200903180670A及201103023）

摘　　要：目的研究中央型记忆T细胞（TCM）和效应型记忆T细胞（TEM）在肝癌肿瘤浸润淋巴细胞中的分布特点。方法通过流式细胞仪检测肝脏组织浸润淋巴细胞CD3、CIM、CD8、CIM5RO、CD62L、CCR7表面标志的表达情况。结果乙肝肝癌组、乙肝癌旁组及对照组CD4＋TCM亚群占CD4＋CD45RO＋Tm的比例分别为（0．25±0．17）％、（0．29±0．22）％、（0．37±0．33）％，差异无显著统计学意义（P〉0．05）。CD4＋TEM亚群占CD4＋CD45RO＋Tm的比例分别为（89．7±7．4）％、（98．7±0．6）％、（95．5±3．7）％，差异有统计学意义（P〈0．05）。其中乙肝肝癌组明显低于乙肝癌旁组及对照组。乙肝肝癌组、乙肝癌旁组及对照组CD8＋TCM亚群占CD8＋CD45RO＋Tm的比例分别为（0．41±0．31）％、（0．55±0．60）％、（0．26±0．25）％，其中乙肝癌旁组显著高于对照组，差异有统计学意义（P〈0．05）。CD8＋TEM亚群占CD8＋CIM5RO＋Tm的比例分别为（92．4±4．3）％、（98．1±0．7）％、（98．2±1．1）％，乙肝肝癌组显著低于乙肝癌旁组及对照组，差异有统计学意义（P〈0．05）。结论原发性肝癌患者肿瘤浸润CD45RO＋Tm中，无论是CD4^＋还是CD8＋，主要以TEM亚群为主，TCM亚群所占比例很小。CD4＋TEM亚群及CD8＋TEM亚群占CIM5RO＋Tm的比例在肝癌组织中显著小于非肝癌组织，而CD4^＋TCM亚群及CD8^＋TCM亚群占CIM5RO＋Tm的比例在肝癌组织及非肝癌组织中无差别。Objective To illustrate the distribution of two memory T cells subsets CD45RO＋ CD62L＋CCR7＋T cells （ central memory T cells, TCM ） and CD45RO＋ CD62L- CCR7- T cells （ effector memory T cells, TEM ） in tumor infiltrating lymphocytes （TILs） of hepatocellular carcinoma （HCC） patients. Methods Polychromatic flow cytometry （PFC） was used to test the cell surface molecules of infil- trating lymphocytes in liver, including CD3, CD4, CDS, CD45RO, CD62L and CCR7. Results The pro-portions of CD4＋ TCM subsets in CD4＋ CD45RO ＋ Tm in HCC tissues, adjacent liver tissues and normal liver tissues were （0.25±0. 17）%, （0.29±0.22）% and （0.37±0.33）%, and there were no significant differences （P 〉 0.05 ）. The proportions of CD4 ＋ TEM subsets in CD4＋ CD45RO＋ Tm in HCC tissues, adjacent liver tissues and normal liver tissues were （89. 7±7. 4） % , （98.7±0. 6） % and （95.5±3.7） %, respectively. Paired comparison showed that the proportions of CD4＋ TEM subsets in HCC tissues were significantly lower than those in adjacent liver tissues and normal liver tissues （ P 〈 0.05 ）. The proportions of CD8 ＋ TCM subsets in CD8＋ CD45RO＋ Tm in HCC tissues, adjacent liver tissues and normal liver tissues were （ 0.41±0.31 ） %, （0.55±0. 60） % and （0.26±0.25 ） %. Paired comparison showed that the proportions of CD8＋TCM subsets in adjacent liver tissues were significantly higher than those in normal liver tissues （ P 〈 0.05 ）. The proportions of CD8＋ TEM subsets in CD8＋ CD45RO＋Tm from HCC tissues, adjacent liver tissues and normal liver tissues were （92.4 ± 4. 3 ） %, （98.1 ± 0. 7 ） % and （98.2±1.1 ） %. Paired comparison showed that the proportions of CD8 -TEM subsets in HCC tissues were significantly lower than those in adja- cent liver tissues and in normal tissues （P 〈 0.05）. Conclusions Comparing to the TCM, TEM subsets including CD4＋ and CD8＋, accounted for the majority of tumor infiltrating CD45RO＋ Tm in HCC p

关 键 词：中央型记忆T细胞 效应型记忆T细胞 肿瘤浸润淋巴细胞 原发性肝癌 

分 类 号：R735.7[医药卫生—肿瘤]