人乳腺癌肿瘤移植裸鼠模型的构建及其肿瘤浸润髓系细胞的分离  

Construction of human breast cancer tumor transplantation model in nude mice and isolation of tumor infiltrating myeloid cells

作　　者：王兴[1] 柳迪[1] 唐丽[1] 贺福初[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所，蛋白质组学国家重点实验室,北京102206

出　　处：《军事医学》2016年第7期561-563,568,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(31570901;31270953);国家973计划资助项目(2013CB910802;2014CBA02000)

摘　　要：目的人乳腺癌肿瘤移植裸鼠模型的构建及其肿瘤浸润髓系细胞的分离。方法将p HAGE-EF-Zs GDEST质粒与病毒包装质粒p MD2.G和ps PAX2首先通过磷酸钙转染方式包装带绿色荧光的慢病毒,再利用包装好的慢病毒感染BT474细胞,通过流式分选筛选阳性细胞,扩大培养后,种植于裸鼠乳腺脂肪垫,应用活体荧光成像仪和体外测量肿瘤大小,对人-裸鼠肿瘤移植模型的构建效果进行评价;通过机械法分离该小鼠模型的肿瘤浸润髓系细胞。结果筛选出带绿色荧光的BT474稳定株,种植于裸鼠乳腺脂肪垫,构建了人-裸鼠肿瘤移植模型,并分离出其肿瘤浸润髓系细胞。结论成功分离出了肿瘤浸润髓系细胞,为后续研究肿瘤浸润髓系细胞的功能奠定了基础。Objective To establish neoplasm transplantation models of breast cancer cells in BALB/c nude mice and to isolate tumor infiltrating myeloid cells. Methods p HAGE-EF-Zs G-DEST plasmid,p MD2. G plasmid and ps PAX2 were transfected into BT474 using the method of calcium phosphate transfection. The positive cells were selected by flow cytometry and implanted in the fat pad of nude mice. A tumor model of breast cancer cells implanted in nude mice was constructed,and the tumor infiltrating myeloid cells were isolated. Conclusion Tumor infiltrating myeloid cells are successfully isolated,which will contribute to the study of the functions of tumor infiltrating myeloid cells.

关 键 词：乳腺肿瘤 肿瘤移植 鼠 模型 肿瘤浸润 髓系细胞 慢病毒 

分 类 号：R737.9[医药卫生—肿瘤] R73-35[医药卫生—临床医学]