山苍子油及其分别与酮康唑、伊曲康唑及氟康唑联合抗马拉色菌的体外药敏试验  

In vitro susceptibility study of litsea cubeba oil and its combination with ketoconazole, itraconazole and fluconazole against Malassezia

作　　者：黄云丽[1] 李红宾[1] 许雪[1] 李玉叶[1] 董天祥[1] 

机构地区：[1]昆明医科大学第一附属医院皮肤科,云南昆明650031

出　　处：《临床皮肤科杂志》2016年第8期565-568,共4页Journal of Clinical Dermatology

基　　金：云南省应用基础研究面上项目(2014FB030);国家自然科学基金(81160076)资助项目

摘　　要：目的:研究山苍子油是否具有抗马拉色菌的作用,明确山苍子油分别与3种抗真菌药物氟康唑、伊曲康唑及酮康唑联合抗真菌效力。方法:参照美国国家临床试验标准化委员会(NCCLS)提出的M27-A方案,测定山苍子油对临床分离的87株优势菌株的最小抑菌浓度(MIC)值,同时测定山苍子油分别与3种抗真菌药物联合应用的MIC值,并比较3种抗真菌药物与山苍子油联合用药前、后的MIC值。结果:山苍子油对临床分离的87株马拉色菌MIC均值为(3130.76±846.75)mg/L,糠秕马拉色菌的MIC均值明显低于合轴马拉色菌和厚皮马拉色菌(P<0.05);山苍子油分别与酮康唑、伊曲康唑及氟康唑联合用药的MIC值比单用时显著降低(P均<0.01)。结论:山苍子油对马拉色菌可能有一定的抗真菌作用;不同马拉色菌对唑类药物的敏感性有差异;山苍子油能够增强3种唑类药物的抗真菌效果。Objective： To investigate the effect of litsea cubeba oil and its combination with ketoconazole, itraconazole and fluconazole against Malassezia. Methods： According to the M27-A protocol established by National Committee For Clinical Laboratory Standards（NCCLS）, we measured the minimum inhibitory concentration（MIC） of litsea eubeba oil and its combi- nation with ketoconazole, itraconazole, and fluconazole in 87 clinical isolates. Results： All of 87 isolates were sensitive to lit- sea cubeba oil with a MIC of 3130.76±846.75 mg/L. Interestingly, the MIC for M. furfur was significantly lower than that for M. sympodialis and M. pachydermatis（P〈0.05）. In comparison with litsea cubeba oil alone, the combination of litsea cubeba oil with any of the three azole antifungal agents lowered MIC（P〈0.01）. Conclusions： We confirm that litsea cubeba oil has antifungal effects. The sensitivity of Malassezia varies with species of fungi and antifungal agents. Litsea cubeba oil could en- hance the antifungal effect of azole antifungal agents.

关 键 词：马拉色菌 药敏试验 山苍子油 抗真菌作用 

分 类 号：R751[医药卫生—皮肤病学与性病学]