虎杖苷对大鼠神经病理性痛的影响  

Effect of polydatin on neuropathic pain in rats

作　　者：赵永忠[1] 杨晓金[1] 马树勇[1] 宋文学[1] 陈旭[1] 王进文[1] 

机构地区：[1]甘肃省酒泉市人民医院手术麻醉科,735000

出　　处：《中华麻醉学杂志》2016年第5期591-594,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价虎杖苷对大鼠神经病理性痛的影响。方法雄性SD大鼠40只，6～8周龄，体重200～230 g，采用随机数字表法分为5组（n＝8）：假手术组（S组）、神经病理性痛组（NP组）、虎杖苷5 mg/kg组（P1组）、虎杖苷10 mg/kg组（P2组）和虎杖苷20 mg/kg组（P3组）。NP组和P1-3组采用坐骨神经慢性压迫性损伤法制备大鼠神经病理性痛模型。S组只暴露坐骨神经，但不结扎。S组和NP组分别于术毕即刻及术后1、3、5、7 d时腹腔注射生理盐水0.1 ml；P1-3组分别于术毕即刻及术后1、3、5、7 d时腹腔注射虎杖苷5、10、20 mg/kg（溶于0.1 ml生理盐水中）。分别于术前1 d（T0）及术后1、3、5、7 d（T1-4）时测定机械缩足反应阈（MWT）和热缩足潜伏期（TWL）。于T4时痛阈测定结束后，断头处死大鼠，取L4-6节段脊髓组织，采用Western blot法测定高迁移率族蛋白1（HMGB1）、Toll样受体4（TLR4）、IL-1β、TNF-α和单核细胞趋化蛋白1（MCP-1）的表达水平。结果与S组比较，NP组T1-4时MWT降低，TWL缩短，P1组T1-4时MWT降低，T2-4时TWL缩短，P2组T1-4时MWT降低，T3，4时TWL缩短，P3组T1-4时MWT降低，NP组、P1组和P2组脊髓HMGB1、TLR4、IL-1β、TNF-α、MCP-1的表达上调（P〈0.05）；与NP组比较，P2组T1-4时MWT升高，T1，2时TWL延长，P3组T1-4时MWT升高，TWL延长，P2组和P3组脊髓HMGB1、TLR4、IL-1β、TNF-α、MCP-1的表达下调（P〈0.05），P1组上述各指标差异无统计学意义（P〉0.05）；与P1组比较，P2组T4时MWT升高，P3组T1-4时MWT升高，T3，4时TWL延长，脊髓HMGB1、TLR4、IL-1β、TNF-α、MCP-1的表达下调（P〈0.05）；与P2组比较，P3组T3，4时MWT升高，脊髓TLR4、IL-1β、TNF-α、MCP-1的表达下调（P〈0.05）。结论虎杖苷可通过抑制脊髓炎症反应减轻大鼠神经病理性痛。Objective To evaluate the effect of polydatin on neuropathic pain in rats. Methods Forty male Sprague-Dawley rats, aged 6- 8 weeks, weighing 200-230 g, were randomly divided into 5 groups （n= 8 each） using a random number table： sham operation group （ group S） , neuropathic pain group （group NP ） , polydatin 5 mg/kg group （ group P1 ） , polydatin 10 mg/kg group （ group P2 ） , and polydatin 20 mg/kg group （group P3 ）. Neuropathic pain was induced by chronic constriction injury in NP and P1-3 groups. In group S, the sciatic nerve was only exposed but not ligated. In S and NP groups, normal saline 0. 1 ml was injected intraperitoneally immediately after operation and at 1, 3, 5 and 7 days after operation （T1_4）. In P1-3 groups, polydatin 5, 10 and 20 mg/kg （in normal saline 0. 1 ml） were injected intraperitoneally immediately after operation and at T1-4. At 1 day before operation （To） and T1-4, the me- chanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured. After measurement of pain threshold at T4 , the rats were sacrificed, and L4_6 segments of the spinal cords were removed for determination of the expression of high-mobility group box 1 （HMGB1） , Toll-like receptor 4 （TLR4） , interleukin-lbeta （IL-1β） , tumor necrosis factor-alpha （TNF-α） and monocyte chemotactie protein-1 （MCP-1） by Western blot. Results Compared with group S, the MWT was significantly de-creased, and the TWL was significantly shortened at T1-4 in group NP, the MWT was significantly de- creased at T1-4 , and the TWL was significantly shortened at T2.4 in group Pl, the MWT was significantly de- creased at T1-4, and the TWL was significantly shortened at T3,4 in group P2, the MWT was significantly decreased at T1-4 in group Pa, and the expression of HMGB1, TLR4, IL-1β, TNF-α and MCP-1 was sig- nificantly up-regulated in NP, P1 and P2 groups （P〈0.05）. Compared with group NP, the MWT was sig- nificantly increased at T1.4, and the TWL w

关 键 词：虎杖 神经痛 

分 类 号：R741[医药卫生—神经病学与精神病学]