三叉神经痛模型大鼠三叉神经节神经元中NPR-A表达量的变化  

Variation of NPR-A receptor expression on mouse trigeminal ganglion neurons of trigeminal neuralgia model

作　　者：韩良[1] 崔曼曼[1] 徐文华[1] 刘悦雁[2] 王烈成[3] 王元银[1] 

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,合肥230032 [2]皖西卫生职业学院生理学教研室,六安237000 [3]安徽医科大学基础医学院生理学教研室,合肥230032

出　　处：《安徽医科大学学报》2016年第8期1097-1100,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81271162);安徽省高等学校省级优秀青年人才基金项目(编号:2012SQRL070);安徽省高校省级优秀青年人才基金重点项目(编号:2013SQRL146ZD)

摘　　要：目的检测三叉神经痛(TN)模型大鼠的三叉神经节(TG)神经元中NPR-A受体mRNA表达量的变化。方法采用眶下神经缩窄术和经眶下孔注射肿瘤坏死因子α(TNF-α)将雄性SD大鼠随机分成6组,即对照组1(假手术组)、对照组2(生理盐水注射组)、眶下神经缩窄模型组(ION-CCI组)术后1周和2周以及经眶下孔注射TNF-α组(TNF-α组)注射后1周和2周。Q-PCR法检测NPR-A受体mRNA的表达。结果每组大鼠TG均表达NPR-A受体mRNA。其中ION-CCI组术后2周以及TNF-α组注射后1周和2周TG内NPR-A受体mRNA表达含量均显著高于相应对照组(n=4,P<0.05)。结论大鼠TG表达的NPR-A受体可能与大鼠TN及炎性痛的发生和发展相关。Objective To investigate the expression of NPR-A receptor on mouse trigeminal ganglions of trigemiual neuralgia model. Methods Male SD rats were randomly assigned into 6 groups ： control group 1 （ sham group ） , control group 2 （ infraorbital foramen inject normal saline group）, chronic constriction injury of the infraorbital nerve guoup（ION-CCI group） 1 week and 2 weeks and infraorbital foramen inject TNF-α group（TNF-α group） 1 week and 2 weeks. The expressions of NPR-A mRNA were detected by Q-PCR. Results The expression of NPR-A was detected in all groups. And the expressions of NPR-A in the ION-CCI group 2 weeks and TNF-α group 1 week and 2 weeks were higher than that in the corresponding control group. Conclusion NPR-A receptor on mouse trigeminal ganglions may be involved in the trigeminal neuralgia and inflmmatory pain.

关 键 词：三叉神经节 慢性压迫性损伤 三叉神经痛 

分 类 号：R745.7[医药卫生—神经病学与精神病学]