受体相互作用蛋白激酶1在食管鳞状细胞癌中的表达及临床意义  

Receptor interacting protein kinase 1 expression in esophageal squamous carcinoma and clinical significance

作　　者：崔凯[1] 耿慧武[2] 姬强[2] 朱克超[1] 卢晨[1] 夏杰[3] 刘晓颖[2] 于在诚[1] 

机构地区：[1]安徽医科大学第一附属医院胸外科,合肥230022 [2]安徽医科大学生命科学学院生物学系,合肥230032 [3]安徽医科大学第四附属医院急诊外科,合肥230011

出　　处：《安徽医科大学学报》2016年第8期1175-1179,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金青年基金(编号:81201368)

摘　　要：目的探讨受体相互作用蛋白激酶1(RIP1)蛋白在食管鳞癌中的表达情况及临床意义。方法应用Western blot法和免疫组织化学法检测并且分析100例食管鳞癌患者的癌组织、相应癌旁组织和对应正常黏膜组织中RIP1的表达情况。结果 71%食管鳞癌组织中RIP1蛋白的表达水平高于对应癌旁组织和正常黏膜组织,20%食管鳞癌癌旁组织RIP1蛋白的表达水平高于对应癌组织和正常食管黏膜组织,差异有统计学意义(P<0.05)。食管鳞癌组织中高表达的RIP1蛋白水平与其TNM分期、病理分级、有无淋巴结转移有关(P<0.05),与性别、年龄无关。癌旁组织中高表达的RIP1蛋白水平与肿瘤浸润深度、TNM分期有关(P<0.05),与性别、年龄、有无淋巴结转移及病理分级无关。结论RIP1蛋白在食管鳞癌中的高表达可能与肿瘤的病理分级、TNM分期及预后有关,对于食管鳞癌的发生发展有一定的预测作用。Objective The investigation of the expression and clinical significance of receptor interacting protein kinase 1 （ RIP1 ） in esophageal squamous cell carcinoma. Methods Immunohistochemistry and Western blot analysis were applied to detect and analyze the expression of RIP1 in cancer tissue and the relevant pericarcinomatous tissue, as well as in the corresponding normal esophageal mucosa in 100 cases of ESCC patients. Results RIP1 expression levels in 71% of cases were demonstrated to be the highest in cancer tissue, then in pericarcinomatous tissue and normal esophageal mucosa in sequence, with statistical significance（P 〈 0. 05）. 20% of cases indicated that expression of RIP1 in pericarcinomatous tissue relating to esophageal squamous carcinoma was higher than that in cancer tissue and normal esophageal mucosa orderly （ P 〈 0.05 ）. The investigated up-regulated expression of RIP1 in cancer tissue was proved to be relevant with TNM stage, pathologic grade and lymph node metastasis（P 〈 0. 05 ）, while it was not associated with sex and age. The up-regulated expression of RIP1 in pericarcinomatous tissue was suggested to be corresponding to invasion depth and TNM stage （ P 〈 0.05 ）, but not to sex, age lymph node metastasis or pathological grading. Conclusion The up-regulated expression of RIP1 in esophageal squamous carcinoma is supposed to be relevant to pathological grading, TNM stage and prognosis, which also is effective in the genesis and development of esophageal squamous carcinoma.

关 键 词：食管肿瘤 鳞状细胞 免疫组织化学 受体相互作用蛋白激酶1 

分 类 号：R735.1[医药卫生—肿瘤] R341[医药卫生—临床医学]