星点设计-效应面法优化马尾松花粉总黄酮提取工艺  

Optimization of extraction process of flavone from the Pinus massoniana pollen by using central composite design- response surface methodology

作　　者：张珉[1] 孙化鹏[2] 唐效蓉[1] 黄帆[1] 夏合新[1] 许忠坤[1] 

机构地区：[1]湖南省林业科学院,湖南长沙410004 [2]湖南农业大学,湖南长沙410128

出　　处：《食品工业科技》2016年第15期230-233,共4页Science and Technology of Food Industry

基　　金：中央财政林业科技推广项目([2003]TK55);湖南省林业科学院青年科研创新基金项目(2013LQJ10)

摘　　要：采用超声辅助提取法从马尾松花粉中提取总黄酮,通过单因素实验考察乙醇体积分数、提取时间、料液比以及提取次数等4个因素对马尾松花粉总黄酮得率的影响,并采用星点设计-效应面法优化总黄酮提取工艺。结果表明:马尾松花粉总黄酮的最佳提取工艺条件为乙醇体积分数62.5%,提取时间28.8 min,料液比1∶24,提取次数3次。在此优化条件下,马尾松花粉总黄酮的实际得率为1.37%,与理论值(1.32%)较为接近,表明采用星点设计-效应面法优化马尾松花粉总黄酮提取工艺的方法可行。The ultrasound-assisted extraction of flavone from the Pinus massoniana pollen was carried out. The effects of ethanol concentration, extraction time, material-liquid ratio and extraction numbers on the extraction rate of flavone were studied by single factor experiment, and the extraction process of flavone was optimized by the central composite design- response surface methodology. The results showed that, the optimal extraction conditions of flavone from the Pinus massoniana pollen were 62.5% of ethanol concentration, 28.8 minutes of extraction time,1 ： 24 of material- liquid ratio and 3 times of extraction number. Under this optimal condition, the actual extraction rate of fiavone reached 1.37%, which was close to the theoretic value.It was feasible to optimize the extraction process of flavone from the Pinus massoniana pollen by using central composite design-response surface methodology.

关 键 词：马尾松花粉 总黄酮 星点设计-效应面法 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]