内质网应激、miR-375在高糖高脂介导胰岛β细胞凋亡中的作用  

Roles of endoplasmic reticulum stress and miR-375 in apoptosis of pancreatic β cells induced by hyperglycemia and hyperlipidemia

作　　者：吉慧珍 杨静[3] 邢小平[2] 李玉秀[2] 潘慧[2] 孙琦[2] 

机构地区：[1]山西医科大学第一临床医学院,山西太原030001 [2]中国医学科学院北京协和医学院北京协和医院内分泌科卫生部内分泌重点实验室,北京100730 [3]山西医科大学附属第一医院内分泌科,山西太原030001

出　　处：《中国卫生检验杂志》2016年第14期2065-2067,2117,共4页Chinese Journal of Health Laboratory Technology

基　　金：国家重点基础研究发展计划(973计划)(2014CB542300);2011年国家临床重点专科建设项目(WBYZ2011-873)

摘　　要：目的观察不同糖脂浓度时内质网应激及miR-375表达的改变对大鼠胰岛RINm5F细胞增殖、凋亡的影响。方法RINm5F细胞分别培养在含不同糖脂浓度的培养液中,利用CCK-8法检测细胞的增殖活性,实时荧光定量PCR(qt-PCR)检测内质网应激相关基因XBP-1,CHOP以及miR-375的表达,Western blot检测凋亡基因caspase-3以及CHOP蛋白的表达。结果相同葡萄糖浓度时,随着棕榈酸浓度的增加,内质网应激相关基因XBP-1 mRNA、CHOP mRNA以及miR-375的表达水平增高(P<0.05),胰岛β细胞的增殖活性下降(P<0.05),Western blot检测可见CHOP及caspase-3蛋白表达均增加。结论在高糖高脂的作用下,内质网应激的诱导及miR-375的增加与胰岛β细胞的凋亡相关。Objective To investigate the roles of endoplasmic reticulum stress and miR - 375 in the apoptosis and multiplica- tion of RINm5F cells under different concentrations of glucose and fat. Methods RINmSF cells were cultured in vitro under different concentrations of glucose and palmitate acid, The Cell Counting Kit -8 was used to measure the proliferative activity of cells. Quantitative real - time PCR（ qt - PCR） was performed to measure endoplasmic reticulum stress related genes XBP - 1, CHOP and miR - 375 expression, and Western blot was used to measure the expression levels of CHOP protein and caspase - 3 protein. Results Under the condition of the same concentration of glucose, plamitate acid could promote the expression of XBP - 1 mRNA, CHOP, mRNA, miR - 375 （ P 〈 0, 05 ）, and decrease the proliferation activity of pancreatic islet beta cells （ P 〈 0. 05 ） ; Western blot detection showed that CHOP and caspase - 3 protein expression were increased. Conclusion Under the action of hyperglycemia and hyperlipidemia, the induction of endoplasmie retieulum stress and the increase of miR - 375 are related to the apoptosis of pancreatic β cells.

关 键 词：内质网应激 miR-375 胰岛Β细胞 凋亡 

分 类 号：R361.3[医药卫生—病理学]