真胃灌注缺失精氨酸、苏氨酸或组氨酸的混合氨基酸对泌乳山羊乳腺氨基酸代谢的影响  

Effects of Abomasul Infusion of Amino Acid Mixture without Arginine,Threonine and Histidine on Mammary Amino Acid Metabolism of Lactating Goats

作　　者：胡志勇[1] 孙得俸 李继伟[1] 闫振贵[1] 林雪彦[1] 王中华[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《动物营养学报》2016年第7期2032-2044,共13页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家现代农业(奶业)产业技术体系(CARS-37);山东省牛产业技术体系(SDAIT-12-011-06);国家自然科学基金(31372340;31572427)

摘　　要：本试验旨在研究真胃灌注缺失精氨酸(Arg)、苏氨酸(Thr)或组氨酸(His)的混合氨基酸对泌乳山羊乳腺氨基酸代谢的影响。选择4只泌乳中期萨能奶山羊,安装真胃插管(用于灌注氨基酸)、颈动脉和乳静脉血管插管(用于采集血样),在外阴动脉安装血液流量计探头(记录乳腺血流量)。试验羊限饲基础饲粮,满足维持能量和蛋白质需要。真胃灌注葡萄糖和按瘤胃微生物蛋白氨基酸构成配制的混合氨基酸。采用4×4拉丁方试验设计,对照组灌注全混合氨基酸,试验组分别灌注缺失Arg、Thr、His的混合氨基酸;试验共进行4期,每期7 d,前4天为灌注期,后3天为采样期。结果表明:1)单一氨基酸缺失灌注对产奶量和乳蛋白产量均无显著影响(P>0.05)。2)Arg和Thr缺失显著提高了乳腺血流量(P≤0.05),降低了该氨基酸的动脉浓度、静脉浓度,乳腺清除率分别上升25.9%和199.2%;单一氨基酸缺失灌注对该氨基酸乳腺吸收量和乳腺吸收产出比无显著影响(P>0.05)。3)Arg、Thr和His缺失,泌乳转化效率分别提高25.4%(4.02 vs.5.04,P≤0.05)、34.5%(9.09 vs.12.23,P≤0.05)和14.6%(10.51 vs.12.04,0.05<P≤0.10)。结果提示,泌乳山羊通过提高乳腺血流量和乳腺清除率增加了饲粮对乳腺缺失氨基酸的供给,从而提高了缺失氨基酸的泌乳转化效率。The purpose of this study was to determine the effects of abomasul infusion of amino acid（ AA）mixture without arginine（ Arg）,threonine（ Thr） and histidine（ His） on mammary AA metabolism of lactating goats. Four Saanen dairy goats at med-lactation period were selected and installed with abomasul（ for AA infusion）,jugular arterial and breast intravenous fistula cannulas（ for blood sample collection）,as well as probes of blood flow meter at external pudendal artery（ for mammary blood flow recording）. Goats for trial were restricted fed a basal diet to meet energy and protein needs for maintenance. Abomasa were infused with glucose and AA mixed solution,which had same AA composition with ruminal microbial protein. A 4 × 4 Latin square design was used,control group was infused total AA mixture,and trial groups were infused AA mixture without one of Arg,Thr or His,respectively; the trial consisted of 4 stages with 7 d per stage,in each of which the first 4 d were infusion period,and the last 3 d were sampling period. The results show ed as follow s：1） infusion of AA mixture without single AA had no significant effects on milk yield and milk protein yield（ P0.05）. 2） Infusion of AA mixture without Arg or Thr significantly increased mammary blood flow（ P≤0.05）,but reduced the concentrations of the missing AA in artery and vein,and the AA clear rates of mammary glands were increased by 25.9% and 199%; infusion of AA mixture without single AA had no significant effects on absorbed amount to yield of AA of mammary glands（ P〉0. 05）. 3） Infusion AA mixture without Arg,Thr or His increased AA conversion efficiency of lactation of mammary glands by 25. 4%（ 4. 02 vs.5.04,P≤0.05）,34.5%（ 9.09 vs. 12.23,P≤0.05） and 14.6%（ 10. 51 vs. 12. 04,0. 05〈 P≤0. 10）,respectively. The results indicate that dairy goats increase the supplementation of mammary gland lacking AA from diet through increasing mammary blood flow and mammary clear rate,and improve conversion effic

关 键 词：氨基酸 乳腺血流量 泌乳性能 泌乳转化效率 

分 类 号：S826[农业科学—畜牧学]