马铃薯晚疫病菌交配型检测方法比较  

Comparison of Methods for Detecting Mating Type in Phytophthora infestans

作　　者：缪云琴 孟然然[1] 唐唯[1] 杨仙[1] 李灿辉[1] 

机构地区：[1]云南师范大学薯类作物研究所,云南昆明650500

出　　处：《西南农业学报》2016年第7期1525-1529,共5页Southwest China Journal of Agricultural Sciences

基　　金：农业部公益性(农业)行业科研专项(3-20);云南省科技厅(2008CA027;2008PY053和2009BB01)

摘　　要：晚疫病是云南省马铃薯第一大病害,近年在云南省种植区有逐年加重的趋势,从1998年发现晚疫病菌的2种交配型以来,有性生殖的风险也随之增加。文章以2010年来自云南省马铃薯主产区的86个晚疫病菌菌株为材料,以对峙培养检测法检测结果为准,比较分析了CAPs标记和A2特异性DNA片段扩增方法的准确性。研究证实了2种分子标记检测方法均可以准确检测出晚疫病菌A1和A2交配型,但不能区分A2和A1A2交配型。A2交配型占总菌株的82.56%,在云南省所有马铃薯主产区都存在,已成为云南省晚疫病菌群体中的优势交配类型。Potato late blight （PLB） was the most serious potato disease in Yunnan, during the decades and PLB became a dominant and persistent problem of potato production, furthermore. The risk of sexual reproduction was going to higher since both two mating type was found in 1998. This research collected 86 isolates of Phytophthora infestans from the mainly potato planted areas in Yunnan, aimed to compare two PCR methods （ CAPs and A2 specific marker） with the paring culture method. The results confirmed that both of the molecular detection methods could distinguish A1 and A2 mating type, but failed to differentiate between A2 and A1 A2 mating type. Meanwhile, this research also found that isolates with A2 mating type contributed up to 82.56 % among tested isolates, which was becoming the dominant lineage in Yunnan province.

关 键 词：晚疫病菌 交配型 对峙培养 分子标记检测 

分 类 号：S632[农业科学—蔬菜学]