尼卡地平对家兔心肌缺血再灌注损伤后心肌细胞凋亡的影响  

Effects of Nicardipine on Apoptosis in Ischemia Reperfusion Myocardium of Rabbits

作　　者：韩露[1] 张继倬[1] 陈兴华[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院心外科,北京100038

出　　处：《中国医科大学学报》2016年第8期746-749,共4页Journal of China Medical University

摘　　要：目的探讨尼卡地平对家兔心肌缺血再灌注损伤后心肌细胞凋亡的影响。方法 40只新西兰家兔随机分为4组,对照组(A组)、缺血组(B组)、再灌注组(C组)、尼卡地平组(D组)。通过结扎冠状动脉左侧旋支40 min,开放120 min建立缺血再灌注模型。采用磷脂酰丝氨酸外翻分析方法(Annexin V法)检测家兔心肌凋亡情况。结果病理切片提示D组心肌细胞受损情况明显低于B组和C组(P<0.01)。D组心肌细胞凋亡明显少于B组及C组(均P<0.01),但高于A组(P<0.05);D组心肌细胞坏死少于B组及C组(均P<0.01),与A组比较无统计学差异(P>0.05)。结论尼卡地平可以减少家兔缺血再灌注损伤所诱导的心肌细胞凋亡。Objective To investigate the effects of nicardipine on apoptosis in ischemia- reperfusion myocardium of rabbits. Methods Forty New Zealand rabbit were randomly divided into 4 groups：control group（group A）,ischemia group（group B）,ischemia- reperfusion group（group C）,nicardipine treatment group（group D）. Ischemia-reperfusion model was established by left circumflex branch coronary artery ligation for 40 min,followed by 120 min reperfusion. Apoptotic cardiomyocyte was detected using the analysis for the exposure of phosphatidyl- serine（Annexin V）method. Results Myocardial cell damage was lower in D group than that in group B and group C from pathology（P〈0.01）. The myocardial cell apoptosis in group D is less than that of group B and group C（P〈0.01）,but higher than that of group A（P〈0.05）. Myocardial cell necrosis is lower than group B and group C（P〈0.01）,and no difference was found comparing with group A（P〉0.05）. Conclusion Nicardipine horizon can reduce the myocardial cell apoptosis in rabbits with myocardial ischemic reperfusion injury.

关 键 词：凋亡 尼卡地平 缺血再灌注 心肌细胞 ANNEXIN V 

分 类 号：R654.2[医药卫生—外科学]