孕期炎症刺激对子代小鼠脂质代谢及FAT/CD36表达的影响  

Prenatal exposure to lipopolysaccharide results in lipid metabolism and FAT/CD36 expression in mice offspring

作　　者：秦书刚 陈新[1] 贾乙[1] 周见至[1] 苏敏[1] 李晓辉[1] 

机构地区：[1]第三军医大学药学系药物研究所,重庆400038

出　　处：《中国药理学通报》2016年第8期1080-1085,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金面上项目(No 81473260);重庆市自然科学基金重点项目(No cstc2015jcyj BX0093)

摘　　要：目的以FAT/CD36为切入点,研究母体孕期炎症刺激对子代脂质代谢的影响。方法 8周龄C57小鼠,♀♂2∶1合笼配种,d 2♀鼠分笼饲养记为在孕0 d,在孕11 d给予孕鼠一次性腹腔注射脂多糖(LPS)(75μg·kg^(- 1)),对照组注射0.2 m L的生理盐水。分别于子鼠4、8、12周取材(♀鼠取肾周脂肪、♂鼠取附睾周围脂肪),对子代小鼠体重、内脏脂肪重量、脂肪组织和细胞中游离脂肪酸(FFA)、甘油三酯(TG)、FAT/CD36表达量进行检测。结果与NS组相比,LPS组小鼠体重、内脏脂肪重量、脂肪系数明显增高,♂鼠附睾脂肪和血液中TG、FFA的表达量明显升高;各周龄♂鼠脂肪组织和诱导的3T3-L1细胞中FAT/CD36明显上调(P<0.05)。结论母体孕期炎症刺激导致子代小鼠脂肪发育异常,游离脂肪酸(FFA)、甘油三酯(TG)、FAT/CD36的表达量明显升高,其机制可能与FAT/CD36对脂质代谢的调节有关。Aim To explore the effect of prenatal exposureto lipopolysaccharide （LPS） on lipid metabolismin mice offspring from the starting point of FAT /CD 36expression. Methods 8-week old C57 mice mated 2.1 ,then they were caged separately,marked as pregnancyod . The pregnant mice were given single intraperitonealinjection of 75μg·kg^-1 LPS ,and the controlreceived injections of 0.2mL saline. The perirenaladipose of female mice and epididymis adipose of malemice were collected in 4w ,8w ,12w ,respectively.The weight of visceral adipose tissue and the free fattyacid（FFA） and of adipose tissue andFAT /CD 36 of offsprng mice were quantitated. ResultsThe body weight of offsprng of LPS group was alsosignificantly higher than that of N S group, and LPSgroup offspring displayed increased adipose tissue wetweights, the expression of T G and F F A was increasedin LPS group compared with N S . Especially,prenatalexposure to inflammatory stimulation resulted inmarked increase of FAT /CD 36 and abnormal adipocytedevelopment. Conclusions Inflammation induced byprenatal exposure to LPS results in increased bodyweight,adipose coefficient and FAT /CD 36 that mightdevelop into obesity in adult mice. These results arerelevant in that anomalous local adipose tissue andFAT /CD 36 regulation may be an important mechanismunderlying obesity.

关 键 词：孕期炎症刺激 FAT/CD36 FFA TG 脂质代谢紊乱 肥胖 

分 类 号：R-332[医药卫生] R977.6